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CD157 / BST1 ELISA Pair Set, Mouse ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK50319 |
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Mouse CD157 / BST1 ELISA Pair Set
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Order or Inquire for CD157 / BST1 ELISA Pair Set |
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High affinity Mouse CD157 / BST1 ELISA Pair Set |
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Detection limit - 31.25 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Mouse CD157 / BST1 ELISA Pair Set Related Products & Topics
Related Areas:
Proteins:
Antibodies:
Background
CD157, also known as bone marrow sromal cell antigen 1 (BST-1), is a glycosylphosphatidylinositol-anchored ectoenzyme belonging to the NADase/ADP-ribosyl cyclase family. It is expressed in the bone marrow, spleen and thymus in lymphoid organs, and the lung, kidney and heart in non-lymphoid organs. In mouse, CD157 is also expressed on lymphoid progenitors. It has a dual nature behaving both as an ectoenzyme and as a receptor. Cross-linking of CD157 induces intracellular Ca2+ mobilization and re-shaping in neutrophils, thus regulating their adhesive and migratory properties. The CD157-mediated signals promote cell polarization, regulate chemotaxis induced through the high affinity formyl-methionyl-leucyl-phenylalanine (fMLP) receptor and control transendothelial migration. CD157 exhibits homology with CD38 and Aplysia ADP-ribosyl cyclase. Like CD38, it has both ADP-ribosyl cyclase and cADPR hydrolase activities. The CD157 gene is synthenic on 4p15 with CD38, with which it also shares a unique genomic organization. Their conservation in phylogeny is striking evidence for their relevance in the life and death cycle of the cell. CD157 has a specific and crucial role in the regulation of innate immunity during inflammation. The murine CD157 is a useful marker for lymphoid progenitor cells initiating gene rearrangement of their antigen receptors.
