CD157 / BST1 Antibody

CD157 / BST1 Antibody Datasheet

CD157 / BST1 Antibody Product Information

• Immunogen: Recombinant Human CD157 / BST1 Protein (Catalog#10060-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 029
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human CD157 / BST1 (rh CD157 / BST1; Catalog#10060-H08H; Met 1- Lys 292; NP_004325.2).

CD157 / BST1 Antibody Usage Guide

• Specificity: Human CD157 / BST1
• No cross-reactivity in ELISA with

  Mouse BST1

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human BST1 in WB. Using a DAB detection system, the detection limit for Human BST1 is approximately 0.5 ng/lane under non-reducing condition. Use of this antibody under reducing conditions is not recommended.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human BST1. The detection limit for Human BST1 is approximately 0.0049 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD157 / BST1 Antibody Background

Human CD157, also known as bone marrow sromal cell antigen 1(BST-1), is a pleiotropic ectoenzyme which belongs to the CD38 family and to the growing number of leukocyte surface molecules known to act independently as both receptors and enzymes. As a 45-kDa surface glycoprotein with a GPI anchor, the CD157 molecule displays two distinct domains in its extracellular component. The first is implicated in the enzymic activities of the molecule and the second features adhesion/signalling properties. CD157 shares several characteristics with CD38, including a similar amino acid sequence and enzymic functions. Both molecules are involved in the metabolism of NAD+, and the CD157 gene is synthenic on 4p15 with CD38, with which it also shares a unique genomic organization. Their conservation in phylogeny is striking evidence for their relevance in the life and death cycle of the cell.

References

1. Kaisho,T. et al., 1994, Proc. Natl. Acad. Sci. U.S.A. 91 (12): 5325-5329.

2. Yamamoto, K. S. et al., 2002, J. Mol. Biol. 316 (3): 711-723. 

3. Ortolan, E. et al., 2002, Cell Biochemistry and Function. 20 (4): 309-322. 

4. Strausberg, R. L. et al., 2003, Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899-16903.