|IP||0.2-1 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Mouse BST1 was immunoprecipitated using:
Lane A:0.5 mg MCF-7 Whole Cell Lysate
Lane B:0.5 mg 293T Whole Cell Lysate0.5 µL anti-Mouse BST1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-Mouse BST1 rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 36 kDa
Observed band size: 34 kDa
Anti-BST1 rabbit polyclonal antibody at 1:500 dilution
Lane A: Raji Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:36 kDa
Observed band size:36 kDa
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD157, also known as ADP-ribosyl cyclase 2, is an ectoenzyme sharing several characteristics with ADP-ribosyl cyclase CD38. CD157 was originally identified as a bone marrow stromal cell molecule (BST-1) with a glycosylphosphatidylinositol (GPI) anchor to bind to the cell surface. CD157 is prevalently expressed by cells of the myeloid lineage. CD157 could act as a receptor with signal transduction capability. Further, it regulates calcium homeostasis and promotes polarization in neutrophils and mediates superoxide (O2−) production in the human U937 myeloid line.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-CD157 Antibody (APC)||Mouse||Monoclonal||FCM||10060-MM19-A|
|Anti-CD157 Antibody (FITC)||Mouse||Monoclonal||FCM||10060-MM19-F|
|Anti-CD157 Antibody (PE)||Mouse||Monoclonal||FCM||10060-MM19-P|
|Anti-CD157 Antibody (APC)||Mouse||Monoclonal||FCM||10060-MM10-A|
|Anti-CD157 Antibody (FITC)||Mouse||Monoclonal||FCM||10060-MM10-F|
|Anti-CD157 Antibody (PE)||Mouse||Monoclonal||FCM||10060-MM10-P|