Anti-CD157 Antibody

Cat: 50319-RP02
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All CD157 Reagents
CRO Services
Product recommended

All CD157 Reagents
Anti-CD157 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CD157 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse CD157
Immunogen
Recombinant Mouse BST1 protein (Catalog#50319-M08H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse BST1 (rM BST1; Catalog#50319-M08H; NP_033893.2; Met 1-Glu 285). BST1 specific IgG was purified by mouse BST1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD157 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD157 Antibody (Rabbit Polyclonal antibody) Images
Mouse CD157/BST1 Immunoprecipitation(IP) 8232

Mouse BST1 was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

0.5 µL anti-Mouse BST1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse BST1 rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 36 kDa

Observed band size: 34 kDa

Mouse CD157/BST1 Western blot (WB) 13664

Anti-BST1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Raji Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:36 kDa

Observed band size:36 kDa

Anti-CD157 Antibody Alternative Names
Anti-114/A10 Antibody;Anti-A530073F09 Antibody;Anti-BP-3 Antibody;Anti-Bp3 Antibody;Anti-Bsta1 Antibody;Anti-CD157 Antibody;Anti-Ly65 Antibody
CD157 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD157, also known as ADP-ribosyl cyclase 2, is an ectoenzyme sharing several characteristics with ADP-ribosyl cyclase CD38. CD157 was originally identified as a bone marrow stromal cell molecule (BST-1) with a glycosylphosphatidylinositol (GPI) anchor to bind to the cell surface. CD157 is prevalently expressed by cells of the myeloid lineage. CD157 could act as a receptor with signal transduction capability. Further, it regulates calcium homeostasis and promotes polarization in neutrophils and mediates superoxide (O2−) production in the human U937 myeloid line.

Full Name
bone marrow stromal cell antigen 1
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Malavasi F, et al. (2006) CD38 and CD157 as Receptors of the Immune System: A Bridge Between Innate and Adaptive Immunity. Molecular Medicine. 12 (11-12): 334-41.
  • Ortolan E, et al. (2002) CD157, the janus of CD38 but with a unique personality. Cell Biochemistry and Function. 20 (4): 309-22.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD157 Antibody Rabbit Polyclonal ELISA 80351-RP01
    Anti-CD157 Antibody Rabbit Polyclonal ELISA,IHC-P 80351-RP02
    Anti-CD157 Antibody Rabbit Monoclonal ELISA(Cap) 50319-R213
    Anti-CD157 Antibody (APC) Mouse Monoclonal FCM 10060-MM19-A
    Anti-CD157 Antibody Mouse Monoclonal FCM 10060-MM19
    Anti-CD157 Antibody (FITC) Mouse Monoclonal FCM 10060-MM19-F
    Anti-CD157 Antibody (PE) Mouse Monoclonal FCM 10060-MM19-P
    Anti-CD157 Antibody Mouse Monoclonal ELISA,ELISA(Cap) 10060-MM05
    Anti-CD157 Antibody Rabbit Polyclonal ELISA 10060-RP01
    Anti-CD157 Antibody (APC) Mouse Monoclonal FCM 10060-MM10-A
    Anti-CD157 Antibody Rabbit Monoclonal ELISA 10060-R029
    Anti-CD157 Antibody Rabbit Polyclonal ELISA 10060-RP02
    Anti-CD157 Antibody Mouse Monoclonal FCM 10060-MM10
    Anti-CD157 Antibody (FITC) Mouse Monoclonal FCM 10060-MM10-F
    Anti-CD157 Antibody (PE) Mouse Monoclonal FCM 10060-MM10-P
    info info
    添加购物车成功! 添加购物车失败!