CD155 / PVR Antibody

CD155 / PVR Antibody Datasheet

CD155 / PVR Antibody Product Information

• Immunogen: Recombinant Mouse CD155 / PVR protein (Catalog#50259-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 501
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD155 / PVR (rM CD155 / PVR; Catalog#50259-M08H; NP_081790.1; Met 1-Arg 345).

CD155 / PVR Antibody Usage Guide

• Specificity: Mouse CD155 / PVR
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse PVR in WB. Using a DAB detection system, the detection limit for Mouse PVR is approximately 5 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse PVR. The detection limit for Mouse PVR is approximately 0.078 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD155 / PVR Antibody Background

CD155, also known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5), is a type I transmembrane single-span glycoprotein, and belongs to the nectins and nectin-like (Necl) subfamily of immunoglobulin superfamily. This protein contains three Ig-like extracellular domains, a transmembrane segment, and a cytoplasmic tail. In humans, CD155 is predominately expressed by enterocytes and cells of gastrointestinal lymphatic tissues and originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV), an etiologic agent of the central nervous system disease poliomyelitis. The normal cellular function of CD155 maybe the involvement of intercellular adhension between epithelial cells. Alternate splicing of the CD155 mRNA yields four different isoforms (α, β, γ, and δ) with the identical extracellular domains. CD155-β and-γ are secreted, whereas the membrane-bound CD155-α and -δ serve as PV receptors. In addition, it has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhension, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.

References

1. Mendelsohn, C.L. et al., 1989, Cell. 56: 855-865.
2. He,Y. et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97: 79-84.
3. Simon, K. et al., 2001, J. Virol. 75: 4984-4989.
4. Minami,Y., et al., 2007, J. Biol. Chem. 282: 18481-18496.
5. Fuchs, A. et al., 2004, J. Immunol. 172: 3994-3998.
6. Tahara-Hanaoka,S. et al., 2006, Blood. 107: 1491-1496.