CD147 / EMMPRIN / Basigin Antibody ( FITC )

FITC Rabbit Anti-Human CD147 / Basigin Antibody

CD147 / Basigin Antibody Product Information

• Immunogen: Recombinant human CD147 protein ( Catalog#10186-H08H )
• Reagents: FITC-conjugated rabbit monoclonal antibody
• Clone ID: 125
• Ig Type: Rabbit IgG
• Concentration: 5 μl/Test, 0.2 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.1% sodium azide
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human CD147 / Basigin ( rh CD147 ; Catalog#10186-H08H ; NP_940991.1 ; Met 1 - His 205 ) and conjugated with FITC under optimum conditions, the unreacted FITC was removed

CD147 / Basigin Antibody Usage Guide

• Specificity: Human CD147 / Basigin / BSG
• Flow Cytometry: Fig 1, Fig 2 and Fig 3. Flow cytometric analysis of anti-CD147 reactivity Profile of anti-CD147 reactivity on HeLa cells, PC-3 cells, A549 cells analyzed by flow cytometry
  

  Fig 1. Analysis of anti-CD147 reactivity on HeLa cells

  

  Fig 2. Analysis of anti-CD147 reactivity on PC-3 cells

  

  Fig 3. Analysis of anti-CD147 reactivity on A549 cells

  Fig 4. Profile of peripheral blood lymphocytes analyzed by flow cytometry
  The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.
  

  Fig 4. Analysis of peripheral blood lymphocytes

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Immunochemistry: ICC/IF: 1-10 μg/mL

  

  Immunofluorescence staining of human CD147 in HeLa cells with rabbit monoclonal antibody (5 µg/mL). The image showing membrane staining of HeLa cells. Primary antibody (FITC conjugated) was incubated for 1 hour at 37℃.

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CD147 in WB.Using a DAB detection system, the detection limit for Human CD147 is approximately 0.25 ng/lane under non-reducing conditions and 5 ng/lane under reducing conditions.
• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

CD147 / Basigin Antibody Background

CD147, also known as Basigin, BSG, and EMMPRIN, is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. CD147 is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation, and tumor progression. Enriched on the plasma memebrane of many human cancer (breast cancer, lymphoma, oral squamous cell carcinoma, glioma, melanoma, lung, bladder and kidney carcinomas ) cells, CD147 induces the production and release of matrix metalloproteinases in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction.

References

1. Kanekura, T. et al., 1991, Cell. Struct. Funct. 16 : 23-30.
2. Muramatsu ,T. et al., 2003, Histol Histopathol. 18 : 981-7.
3. Jin-Ming Yang. et al., 2006, J. Biol. Chem. 281: 9719-27.
4. Hakuma, N. et al., 2007, Oncology. 72 : 197-204.
5. Zheng HC. et al., 2006, Br. J. Cancer. 95 : 1371-8.
6. Yurchenko,V. et al., 2002, J. Biol. Chem. 277 : 22959-65.