CD147 / EMMPRIN / Basigin antibody (Antigen Affinity Purified)

Mouse CD147 / Basigin / BSG Antibody ( Antigen Affinity Purified )

CD147 / Basigin / BSG Antibody Product Information

• Immunogen: Recombinant mouse CD147 protein ( Catalog#50332-M08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant mouse BSG / CD147 extracellular domain ( rM BSG / CD147 ; Catalog#50332-M08H ; NP_001070652.1 ; Met 1 - Arg 209 ). BSG / CD147 specific IgG was purified by mouse CD147 affinity chromatography.

CD147 / Basigin / BSG Antibody Usage Guide

• Specificity: Mouse Basigin / BSG / CD147
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect mouse CD147 in WB. Using a DAB detection system, the detection limit for mouse CD147 is approximately 0.5 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.5 -1.0 μg/mL with the appropriate secondary reagents to detect mouse CD147. The detection limit for mouse CD147 is 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD147 / Basigin / BSG Antibody Background

Mouse CD147, also known as BSG or EMMPRIN, is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. CD147 is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including sperm-atogenesis, embryo implantation, neural network formation, and tumor progression. Enriched on the plasma memebrane of many human cancer ( breast cancer, lymphoma, oral squamous cell carcinoma, glioma, melanoma, lung, bladder and kidney carcinomas ) cells, CD147 induces the production and release of matrix metalloproteinases in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. CD147 is a key molecule related to mouse blastocyst implantation. The high level of CD147 expression in the blastocyst may also play a role during embryo implantation.

References

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6. Ding, NZ. et al., 2002, Zygote. 10 (3): 239-43.