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CD147 / EMMPRIN / Basigin Antibody, Mouse MAb

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Human CD147/BSG Antibody Product Information
Immunogen:Recombinant Human CD147 protein (Catalog#10186-H08H)
Clone ID:6H11C1
Ig Type:Mouse IgG2a
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD147. (rh CD147; Catalog#10186-H08H; NP_940991.1; Met 1-His 205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human CD147/BSG Antibody Usage Guide
Specificity:Human CD147 / Basigin / BSG
No cross-reactivity with Human cell lysate (293 cell line) in WB and ELISA.
Application:ELISA, IHC-P

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CD147. The detection limit for Human CD147 is 0.0195 ng/well.

IHC-P: 5-20 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CD147/BSG Antibody IHC Application Image
CD147 / EMMPRIN / Basigin Antibody, Mouse MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD147 in human esophagus with mouse monoclonal antibody (10 µg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of squamous epithelium cell.
CD147 / EMMPRIN / Basigin Antibody, Mouse MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD147 in human gastric cancer with mouse monoclonal antibody (10 µg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.
CD147 / EMMPRIN / Basigin Antibody, Mouse MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD147 in human stomach with mouse monoclonal antibody (15 µg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.
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Reactivity : Human  
Application : ELISA  WB  
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Reactivity : Human  
Application : FCM  IF  ICC/IF  
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10186-R125F-50
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Application : ELISA  WB  IHC-P  FCM  IF  IP  IHC-Fr  ICC/IF  
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Reactivity : Human  
Application : FCM  
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10186-R125-P-50
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Reactivity : Human  
Application : FCM  IF  ICC/IF  
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10186-MM08-F-50
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Reactivity : Human  
Application : FCM  
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10186-MM08-A-100
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Reactivity : Human  
Application : ELISA  FCM  IF  ICC/IF  
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10186-MM08-50
10186-MM08-100
10186-MM08-200
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100 µg 
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Reactivity : Human  
Application : FCM  
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10186-MM08-P-100
10186-MM08-P-50
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Reactivity : Human  
Application : ELISA  IHC-P  
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10186-RP02-50
10186-RP02-100
10186-RP02-200
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100 µg 
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Reactivity : Mouse  
Application : ELISA  
    50332-RP02-200
    50332-RP02-50
    50332-RP02-100
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    Reactivity : Mouse  
    Application : ELISA  IF  ICC/IF  
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    50332-R041-50
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    Reactivity : Mouse  
    Application : ELISA  
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      50332-RP01-200
       
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      CD147/EMMPRIN/Basigin Background

      CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.

      Human CD147/EMMPRIN/Basigin References
    • Tang Y, et al. (2004) Tumor-stroma interaction: positive feedback regulation of extracellular matrix metalloproteinase inducer (EMMPRIN) expression and matrix metalloproteinase-dependent generation of soluble EMMPRIN. Mol Cancer Res. 2(2): 73-80.
    • Wilson MC, et al. (2005) Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70). J Biol Chem. 280(29): 27213-21.
    • Curtin KD, et al. (2005) Basigin (EMMPRIN/CD147) interacts with integrin to affect cellular architecture. J Cell Sci. 118(Pt 12): 2649-60.
    • Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44. Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44.
    • Seizer P, et al. (2009) EMMPRIN (CD147) is a novel receptor for platelet GPVI and mediates platelet rolling via GPVI-EMMPRIN interaction. Thromb Haemost. 101(4): 682-6.
    • Moonsom S, et al. (2010) A Competitive ELISA for Quantifying Serum CD147: Reduction of Soluble CD147 Levels in Cancer Patient Sera. Hybridoma (Larchmt). 29(1): 45-52.
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      Catalog: 10186-MM05-50
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      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"