Anti-CD147 Antibody

Cat: 10186-MM05
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All CD147 Reagents
Anti-CD147 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-CD147 Antibody
Validated applications
ELISA,ELISA(Cap),IHC-P
Species reactivity
Reacts with: Human
Specificity
Human CD147
Immunogen
Recombinant Human CD147 protein (Catalog#10186-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD147. (rh CD147; Catalog#10186-H08H; NP_940991.1; Met 1-His 205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2a Clone #6H11C1
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD147 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
IHC-P 1:50-1:200  
ELISA(Cap) 1:250-1:2000 This antibody will detect Human CD147 / EMMPRIN / Basigin in ELISA pair set (Catalog: # SEK10186). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 10186-R118).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD147 Antibody (Mouse Monoclonal antibody) Images
Human CD147/EMMPRIN/Basigin Immunohistochemistry(IHC) 4566
Immunochemical staining of human CD147 in human stomach with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.
Human CD147/EMMPRIN/Basigin Immunohistochemistry(IHC) 4571
Immunochemical staining of human CD147 in human gastric cancer with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.
Human CD147/EMMPRIN/Basigin Immunohistochemistry(IHC) 4574
Immunochemical staining of human CD147 in human esophagus with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). The image showing membrane staining of squamous epithelium cell.
Anti-CD147 Antibody Alternative Names
Anti-5F7 Antibody;Anti-Basigin Antibody;Anti-CD147 Antibody;Anti-EMMPRIN Antibody;Anti-M6 Antibody;Anti-OK Antibody;Anti-TCSF Antibody
CD147 Background Information

CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.

Full Name
basigin (Ok blood group)
References
  • Tang Y, et al. (2004) Tumor-stroma interaction: positive feedback regulation of extracellular matrix metalloproteinase inducer (EMMPRIN) expression and matrix metalloproteinase-dependent generation of soluble EMMPRIN. Mol Cancer Res. 2(2): 73-80.
  • Wilson MC, et al. (2005) Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70). J Biol Chem. 280(29): 27213-21.
  • Curtin KD, et al. (2005) Basigin (EMMPRIN/CD147) interacts with integrin to affect cellular architecture. J Cell Sci. 118(Pt 12): 2649-60.
  • Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44. Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44.
  • Seizer P, et al. (2009) EMMPRIN (CD147) is a novel receptor for platelet GPVI and mediates platelet rolling via GPVI-EMMPRIN interaction. Thromb Haemost. 101(4): 682-6.
  • Moonsom S, et al. (2010) A Competitive ELISA for Quantifying Serum CD147: Reduction of Soluble CD147 Levels in Cancer Patient Sera. Hybridoma (Larchmt). 29(1): 45-52.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD147 Antibody Rabbit Monoclonal ELISA,ICC/IF,IF 50332-R041
    Anti-CD147 Antibody Rabbit Polyclonal ELISA 50332-RP01
    Anti-CD147 Antibody Rabbit Polyclonal ELISA 50332-RP02
    Anti-CD147 Antibody (PE) Mouse Monoclonal FCM 10186-MM08-P
    Anti-CD147 Antibody Rabbit Monoclonal WB,ELISA,IHC-P,IHC-Fr,FCM,ICC/IF,IF,IP 10186-R125
    Anti-CD147 Antibody (APC) Mouse Monoclonal FCM 10186-MM08-A
    Anti-CD147 Antibody Rabbit Monoclonal ELISA(Det) 10186-R118
    Anti-CD147 Antibody (FITC) Rabbit Monoclonal FCM,ICC/IF,IF 10186-R125-F
    Anti-CD147 Antibody (PE) Rabbit Monoclonal FCM 10186-R125-P
    Anti-CD147 Antibody Rabbit Polyclonal ELISA,IHC-P 10186-RP02
    Anti-CD147 Antibody Mouse Monoclonal ELISA,FCM,ICC/IF,IF 10186-MM08
    Anti-CD147 Antibody (FITC) Mouse Monoclonal FCM,ICC/IF,IF 10186-MM08-F
    Anti-CD147 Antibody (APC) Rabbit Monoclonal FCM 10186-R125-A
    Anti-CD147 Antibody Rabbit Polyclonal WB,ELISA 10186-RP01
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