Anti-CD147 Antibody

Anti-CD147 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-CD147 Antibody
Validated applications
WB,ELISA,IHC-P,IHC-Fr,FCM,ICC/IF,IF,IP
Species reactivity
Reacts with: Human
Specificity
Human CD147
Immunogen
Recombinant Human CD147 protein (Catalog#10186-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD147 / Basigin (rh CD147; Catalog#10186-H08H; NP_940991.1; Met 1-His 205).
Source
Monoclonal Rabbit IgG Clone #125
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD147 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:2500-1:10000
IHC-Fr 1:500-1:2000
ICC-IF 1:50-1:1000
FCM 1:25-1:100
IP 4-6 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD147 Antibody (Rabbit Monoclonal antibody) Images

Immunofluorescence staining of human CD147 in HeLa cells with rabbit monoclonal antibody (1:200). The image showing membrane staining of HeLa cells.
Immunofluorescence staining of CD147 in monkey stomach with rabbit monoclonal antibody (1:1000, frozen section). The image showing membrane staining of gastric gland cell. The right panel: merge with DAPI
Immunochemical staining of human CD147 in human stomach with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.
Immunochemical staining of human CD147 in human rectal cancer with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell in intestinal gland.
Immunochemical staining of human CD147 in human placenta with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing positive staining of trophoblast.
Immunochemical staining of human CD147 in human liver with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of hepatocyte.
Immunochemical staining of human CD147 in human gastric cancer with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.
Immunochemical staining of human CD147 in human esophagus with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of squamous epithelium cell. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Analysis of anti-CD147 reactivity on HeLa cells.

CD147 was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

2 µL anti-CD147 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD147 rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 42 kDa

Observed band size: 50 kDa

Anti-CD147 rabbit monoclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:42 kDa

Observed band size:54 kDa

Anti-CD147 Antibody: Alternative Names

Anti-5F7 Antibody; Anti-Basigin Antibody; Anti-CD147 Antibody; Anti-EMMPRIN Antibody; Anti-M6 Antibody; Anti-OK Antibody; Anti-TCSF Antibody

CD147 Background Information

CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.
Full Name
basigin (Ok blood group)
References
  • Tang Y, et al. (2004) Tumor-stroma interaction: positive feedback regulation of extracellular matrix metalloproteinase inducer (EMMPRIN) expression and matrix metalloproteinase-dependent generation of soluble EMMPRIN. Mol Cancer Res. 2(2): 73-80.
  • Wilson MC, et al. (2005) Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70). J Biol Chem. 280(29): 27213-21.
  • Curtin KD, et al. (2005) Basigin (EMMPRIN/CD147) interacts with integrin to affect cellular architecture. J Cell Sci. 118(Pt 12): 2649-60.
  • Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44. Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44.
  • Seizer P, et al. (2009) EMMPRIN (CD147) is a novel receptor for platelet GPVI and mediates platelet rolling via GPVI-EMMPRIN interaction. Thromb Haemost. 101(4): 682-6.
  • Moonsom S, et al. (2010) A Competitive ELISA for Quantifying Serum CD147: Reduction of Soluble CD147 Levels in Cancer Patient Sera. Hybridoma (Larchmt). 29(1): 45-52.
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