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CD147 / EMMPRIN / Basigin Antibody, Rabbit MAb

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Human CD147/BSG Antibody Product Information
Immunogen:Recombinant Human CD147 protein (Catalog#10186-H08H)
Clone ID:125
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human CD147 / Basigin (rh CD147; Catalog#10186-H08H; NP_940991.1; Met 1-His 205).
Human CD147/BSG Antibody Usage Guide
Specificity:Human CD147 / Basigin / BSG
Application:WB, ELISA, IHC-P, ICC/IF, IF, FCM, IP

WB: 5-10 μg/mL

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD147. The detection limit for Human CD147 is 0.00245 ng/well .

IHC-P: 0.1-5 μg/mL

FCM: 0.5-2 μg/Test

IHC-Fr: 0.5-5 μg/mL

ICC/IF: 1-10 μg/mL

IP: 4-6 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CD147/BSG Antibody WB Application Image
Human CD147/BSG Antibody IHC Application Image
CD147 / EMMPRIN / Basigin Antibody, Rabbit MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD147 in human esophagus with rabbit monoclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of squamous epithelium cell. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
CD147 / EMMPRIN / Basigin Antibody, Rabbit MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD147 in human gastric cancer with rabbit monoclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.
CD147 / EMMPRIN / Basigin Antibody, Rabbit MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD147 in human liver with rabbit monoclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of hepatocyte.
CD147 / EMMPRIN / Basigin Antibody, Rabbit MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD147 in human placenta with rabbit monoclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). The image showing positive staining of trophoblast.
CD147 / EMMPRIN / Basigin Antibody, Rabbit MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD147 in human rectal cancer with rabbit monoclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell in intestinal gland.
CD147 / EMMPRIN / Basigin Antibody, Rabbit MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD147 in human stomach with rabbit monoclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.
CD147 / EMMPRIN / Basigin Antibody, Rabbit MAb, Immunofluorescence
[Click to enlarge image]
Caption:
Immunofluorescence staining of CD147 in monkey stomach with rabbit monoclonal antibody (1μg/mL, frozen section). The image showing membrane staining of gastric gland cell. The right panel: merge with DAPI
Human CD147/BSG Antibody IF Application Image
CD147 / EMMPRIN / Basigin Antibody, Rabbit MAb, Immunofluorescence
[Click to enlarge image]
Caption:
Immunofluorescence staining of human CD147 in HeLa cells with rabbit monoclonal antibody (5 µg/mL). The image showing membrane staining of HeLa cells.
Human CD147/BSG Antibody FC Application Image
CD147 / EMMPRIN Antibody, Rabbit MAb, Flow cytometric analysis
[Click to enlarge image]
Caption:
Analysis of anti-CD147 reactivity on HeLa cells.
Human CD147/BSG Antibody IP Application Image
Other CD147/BSG Antibody Products
Reactivity: Human  
Application: FCM  
  • Slide 1
10186-MM08-A-50
10186-MM08-A-100
50 Tests 
100 Tests 
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Reactivity: Human  
Application: ELISA  WB  
  • Slide 1
10186-RP01-100
10186-RP01-200
10186-RP01-400
100 µg 
200 µg 
 
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Reactivity: Human  
Application: ELISA  IHC-P  
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10186-RP02-50
10186-RP02-100
10186-RP02-200
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Reactivity: Human  
Application: ELISA  IHC-P  
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10186-MM05-50
10186-MM05-100
10186-MM05-200
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Reactivity: Human  
Application: FCM  IF  ICC/IF  
  • Slide 1
  • Slide 1
10186-R125F-50
10186-R125F-100
50 Tests 
100 Tests 
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Reactivity: Human  
Application: FCM  IF  ICC/IF  
  • Slide 1
  • Slide 1
10186-MM08-F-50
10186-MM08-F-100
50 Tests 
100 Tests 
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Reactivity: Human  
Application: ELISA  FCM  IF  ICC/IF  
  • Slide 1
  • Slide 1
10186-MM08-50
10186-MM08-100
10186-MM08-200
50 µg 
100 µg 
200 µg 
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Reactivity: Human  
Application: FCM  
  • Slide 1
10186-R125-P-50
10186-R125-P-100
50 Tests 
100 Tests 
Add to Cart
Reactivity: Human  
Application: FCM  
  • Slide 1
10186-MM08-P-100
10186-MM08-P-50
100 Tests 
50 Tests 
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Reactivity: Human  
Application: FCM  
  • Slide 1
10186-R125-A-100
10186-R125-A-50
100 Tests 
50 Tests 
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Reactivity: Mouse  
Application: ELISA  
    50332-RP01-400
    50332-RP01-100
    50332-RP01-200
     
    100 µg 
    200 µg 
    Add to Cart
    Reactivity: Mouse  
    Application: ELISA  
      50332-RP02-200
      50332-RP02-50
      50332-RP02-100
      200 µg 
      50 µg 
      100 µg 
      Add to Cart
      Reactivity: Mouse  
      Application: ELISA  IF  ICC/IF  
      • Slide 1
      50332-R041-50
      50332-R041-100
      50 µg 
      100 µg 
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      CD147/EMMPRIN/Basigin Background

      CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.

      Human CD147/EMMPRIN/Basigin References
    • Tang Y, et al. (2004) Tumor-stroma interaction: positive feedback regulation of extracellular matrix metalloproteinase inducer (EMMPRIN) expression and matrix metalloproteinase-dependent generation of soluble EMMPRIN. Mol Cancer Res. 2(2): 73-80.
    • Wilson MC, et al. (2005) Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70). J Biol Chem. 280(29): 27213-21.
    • Curtin KD, et al. (2005) Basigin (EMMPRIN/CD147) interacts with integrin to affect cellular architecture. J Cell Sci. 118(Pt 12): 2649-60.
    • Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44. Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44.
    • Seizer P, et al. (2009) EMMPRIN (CD147) is a novel receptor for platelet GPVI and mediates platelet rolling via GPVI-EMMPRIN interaction. Thromb Haemost. 101(4): 682-6.
    • Moonsom S, et al. (2010) A Competitive ELISA for Quantifying Serum CD147: Reduction of Soluble CD147 Levels in Cancer Patient Sera. Hybridoma (Larchmt). 29(1): 45-52.
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      Catalog: 10186-R125-50
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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"