Mouse CD146 / MCAM Protein (His Tag)

Melanoma cell adhesion molecule Protein Datasheet

CD146 / MCAM Protein Product Information

• Synonym: 1-gicerin, AV025631, CD146, CD149, Muc18, s-endo, s-gicerin
• Protein Construction: A DNA sequence encoding the mouse MCAM (Q8R2Y2-1) extracellular domain (Met 1-Val 563) was expressed, fused with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

CD146 / MCAM Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Val 24
• Molecular Mass: The secreted recombinant mouse MCAM comprises 551 amino acids and has a calculated molecular mass of 61.3kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 90-100 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD146 / MCAM protein

CD146 / MCAM Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD146 / MCAM Protein Description

The CD146 antigen, also known as MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca²⁺ -independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.

References

1. Qin X. et al., 2001, Mamm Genome. 12 (8): 582-9.
2. Turnberg D. et al., 2004, Am J Pathol. 165 (3): 825-32.
3. Turnberg D. et al., 2003, J Am Soc Nephrol. 14 (9): 2271-9.
4. Sivasankar B. et al., 2007, Mol Immunol. 44 (11): 2978-87.
5. Ramaglia V. et al., 2009, Mol Immunol. 46 (8-9): 1892-6.