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> ELISA > ELISA Pair Set > CD146 / MCAM / MUC18 ELISA Pair Set, Human ELISA Pair Set CD146 / MCAM / MUC18 ELISA Pair Set, Human ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK10115 |
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Human CD146 ELISA Pair Set
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Order or Inquire for Human CD146 ELISA Pair Set |
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High affinity Human CD146 ELISA Pair Set |
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Detection limit - 31.25 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Human CD146 ELISA Pair Set Related Products & Topics
Related Areas:
Proteins:
Antibodies:
Background
Cell surface glycoprotein MUC18, also known as Melanoma-associated antigen MUC18, Cell surface glycoprotein P1H12, CD146 and MCAM, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. The expression of CD146 may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. CD146 is expressed predominantly on metastatic lesions and advanced primary tumours, and has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.
Among molecules at the interendothelial junction, CD146 is involved in cell-cell cohesion and permeability. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. CD146 is regulated by the inflammatory cytokine TNF and that CD146 and soluble form CD146 (sCD146) are both involved in monocyte transendothelial migration during inflammation. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis. CD146 promotes tumor progression of many cancers including melanoma and prostate. Its expression is frequently lost in breast carcinoma cells, and it may act as a suppressor of breast cancer progression. CD146 was originally identified as a biomarker of melanoma, and plays an important role in cancer invasion and metastasis. Further studies revealed that CD146 as a novel endothelial marker was also involved in angiogenesis.
