CD146 / MCAM Antibody ( FITC )

CD146 / MCAM Antibody Datasheet

CD146 / MCAM Antibody Product Information

• Immunogen: Recombinant Human CD146 / MCAM protein (Catalog#10115-H08H)
• Reagents: FITC-conjugated rabbit monoclonal antibody
• Clone ID: 44
• Ig Type: Rabbit IgG
• Concentration: 5 μl/Test, 0.2 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.1% sodium azide
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD146 / MCAM (rh CD146; Catalog#10115-H08H; NP_006491.2; Met 1-Gly 559) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

CD146 / MCAM Antibody Usage Guide

• Specificity: Human CD146 / MCAM
• No cross-reactivity in ELISA with
  

  Human CD66a

  Human CD166

  Human CD106

  Human BCAM

  Human CD226

• Flow Cytometry: Fig 1, Fig 2. Flow cytometric analysis of anti-CD146 reactivity on HeLa cells and A549 cells.

  Profile of anti-CD146 reactivity on HeLa cells, A549 cells analyzed by flow cytometry.

  

  Fig 1. Analysis of anti-CD146 reactivity on HeLa cells

  

  Fig 2. Analysis of anti-CD146 reactivity on A549 cells

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CD146 in WB.
• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

CD146 / MCAM Antibody Background

The CD146 antigen, also known as MCAM, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. CD146 is expressed predominantly on metastatic lesions and advanced primary tumours, and has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.

References

1. Lehmann, JM. et al., 1989, Proc. Natl. Acad. Sci. 86: 9891-5.
2. Shih IM. et al., 1999, J. Pathol. 189: 4-11.
3. Xie S. et al., 1997, Cancer. Res. 57 : 2295-303.
4. Guezguez, B. et al., 2007, J. Immunol. 179: 6673-85.