Anti-CD146 / MCAM Antibody

Cat: 10115-R005
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Anti-CD146 / MCAM Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-CD146 / MCAM Antibody
Validated applications
WB,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human CD146 / MCAM
No cross-reactivity in ELISA with Human CD66a / CEACAM1 Human CD166 / alcama Human CD106 / VCAM1 Human CD226 / DNAM-1 Human BCAM / CD239
Immunogen
Recombinant Human CD146 protein (Catalog#10115-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD146 / MCAM extracellular domain (rh CD146; Catalog#10115-H08H; NP_006491.2; Met 1-Gly 559) .
Source
Monoclonal Rabbit IgG Clone #5
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD146 / MCAM Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
IHC-P 1:500-1:2000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD146 / MCAM Antibody (Rabbit Monoclonal antibody) Images
Human CD146/MCAM Immunohistochemistry(IHC) 2709
Immunohistochemical staining of formalin fixed, paraffin-embedded human stomach showing membrane staining of endothelial cells (red arrow), smooth muscle cells (black arrow) (1:1000).
Human CD146/MCAM Immunohistochemistry(IHC) 2718
Immunohistochemical staining of formalin fixed, paraffin-embedded human lymphonode showing membrane staining of endothelial cells (red arrow), smooth muscle cells (black arrow) (1:1000).
Human CD146/MCAM Immunohistochemistry(IHC) 2727
Immunohistochemical staining of formalin fixed, paraffin-embedded human kidney showing membrane staining of endothelial cells (red arrow) (1:1000).
Human CD146/MCAM Immunoprecipitation(IP) 6139

CD146 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

1 µL anti-CD146 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD146 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 130 kDa

Observed band size: 130 kDa

Human CD146/MCAM Western blot (WB) 6882

Anti-CD146 rabbit monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:72 kDa

Observed band size:115 kDa

Anti-CD146 / MCAM Antibody Alternative Names
Anti-CD146 Antibody;Anti-MUC18 Antibody
CD146 / MCAM Background Information

The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.

Full Name
melanoma cell adhesion molecule
References
  • Mills L, et al. (2002) Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 62(17): 5106-14.
  • Taira E, et al. (2004) Characterization of Gicerin/MUC18/CD146 in the rat nervous system. J Cell Physiol. 198(3): 377-87.
  • Fritzsche FR, et al. (2008) CD146 protein in prostate cancer: revisited with two different antibodies. Pathology. 40(5): 457-64.
  • Bidlingmaier S, et al. (2009) Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 69(4): 1570-7.
  • Boneberg EM, et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 78(3): 325-31.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD146 / MCAM Antibody Rabbit Monoclonal IHC-P 50794-R273
    Anti-CD146 / MCAM Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50794-RP02
    Anti-CD146 / MCAM Antibody Rabbit Polyclonal ELISA 50794-RP01
    Anti-CD146 / MCAM Antibody Rabbit Monoclonal ELISA 50794-R112
    Anti-CD146 / MCAM Antibody Mouse Monoclonal ELISA 10115-MM04
    Anti-CD146 / MCAM Antibody (APC) Mouse Monoclonal FCM 10115-MM01-A
    Anti-CD146 / MCAM Antibody Rabbit Monoclonal ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IF 10115-R044
    Anti-CD146 / MCAM Antibody (PE) Rabbit Monoclonal FCM,ICC/IF,IF 10115-R044-P
    Anti-CD146 / MCAM Antibody (FITC) Rabbit Monoclonal FCM,ICC/IF,IF 10115-R044-F
    Anti-CD146 / MCAM Antibody Rabbit Polyclonal ELISA 10115-RP01
    Anti-CD146 / MCAM Antibody (APC) Rabbit Monoclonal FCM 10115-R044-A
    Anti-CD146 / MCAM Antibody Rabbit Monoclonal WB,ELISA,IHC-P 10115-R012
    Anti-CD146 / MCAM Antibody Mouse Monoclonal FCM 10115-MM01
    Anti-CD146 / MCAM Antibody Rabbit Polyclonal ELISA,IHC-P 10115-RP02
    Anti-CD146 / MCAM Antibody Mouse Monoclonal ELISA,ELISA(Cap),IP 10115-MM05
    Anti-CD146 / MCAM Antibody (PE) Mouse Monoclonal FCM 10115-MM01-P
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