Anti-CD146 / MCAM Antibody

Cat: 10115-MM03
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All CD146 / MCAM Reagents
Anti-CD146 / MCAM Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-CD146 / MCAM Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human CD146 / MCAM
Has cross-reactivity in ELISA with Human CD66a / CEACAM1 Human CD166 / alcama Human CD106 / VCAM1 Human CD226 / DNAM-1 Has cross-reactivityin WB at 100 ng/lane under non-reduced conditions and reduced conditions with Human CD66a / CEACAM1 Human CD166 / alcama Human CD226 / DNAM-1
Immunogen
Recombinant Human CD146 protein (Catalog#10115-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD146 / MCAM extracellular domain (rh CD146; Catalog#10115-H08H; NP_006491.2; Met 1-Gly 559). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #10H1F10G4
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD146 / MCAM Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 0.5-1 μg/mL  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD146 / MCAM Antibody Alternative Names
Anti-CD146 Antibody;Anti-MUC18 Antibody
CD146 / MCAM Background Information

The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.

Full Name
melanoma cell adhesion molecule
References
  • Mills L, et al. (2002) Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 62(17): 5106-14.
  • Taira E, et al. (2004) Characterization of Gicerin/MUC18/CD146 in the rat nervous system. J Cell Physiol. 198(3): 377-87.
  • Fritzsche FR, et al. (2008) CD146 protein in prostate cancer: revisited with two different antibodies. Pathology. 40(5): 457-64.
  • Bidlingmaier S, et al. (2009) Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 69(4): 1570-7.
  • Boneberg EM, et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 78(3): 325-31.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD146 / MCAM Antibody Rabbit Monoclonal IHC-P 50794-R273
    Anti-CD146 / MCAM Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50794-RP02
    Anti-CD146 / MCAM Antibody Rabbit Polyclonal ELISA 50794-RP01
    Anti-CD146 / MCAM Antibody Rabbit Monoclonal ELISA 50794-R112
    Anti-CD146 / MCAM Antibody Mouse Monoclonal ELISA 10115-MM04
    Anti-CD146 / MCAM Antibody (APC) Mouse Monoclonal FCM 10115-MM01-A
    Anti-CD146 / MCAM Antibody Rabbit Monoclonal ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IF 10115-R044
    Anti-CD146 / MCAM Antibody Rabbit Monoclonal WB,IHC-P,IP 10115-R005
    Anti-CD146 / MCAM Antibody (PE) Rabbit Monoclonal FCM,ICC/IF,IF 10115-R044-P
    Anti-CD146 / MCAM Antibody (FITC) Rabbit Monoclonal FCM,ICC/IF,IF 10115-R044-F
    Anti-CD146 / MCAM Antibody Rabbit Polyclonal ELISA 10115-RP01
    Anti-CD146 / MCAM Antibody (APC) Rabbit Monoclonal FCM 10115-R044-A
    Anti-CD146 / MCAM Antibody Rabbit Monoclonal WB,ELISA,IHC-P 10115-R012
    Anti-CD146 / MCAM Antibody Mouse Monoclonal FCM 10115-MM01
    Anti-CD146 / MCAM Antibody Rabbit Polyclonal ELISA,IHC-P 10115-RP02
    Anti-CD146 / MCAM Antibody Mouse Monoclonal ELISA,ELISA(Cap),IP 10115-MM05
    Anti-CD146 / MCAM Antibody (PE) Mouse Monoclonal FCM 10115-MM01-P
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