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CD146 / MCAM Antibody, Rabbit PAb, Antigen Affinity Purified

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Human CD146/MCAM Antibody Product Information
Immunogen:Recombinant Human CD146 protein (Catalog#10115-H08H)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human CD146 / MCAM extracellular domain (rh CD146; Catalog#10115-H08H; NP_006491.2; Met 1-Gly 559). CD146 specific IgG was purified by human CD146 affinity chromatography.
Human CD146/MCAM Antibody Usage Guide
Specificity:Human CD146 / MCAM
Application:ELISA, IHC-P

ELISA: 0.5-1.0 μg/mL

This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human CD146. The detection limit for Human CD146 is 0.00245 ng/well .

IHC-P: 0.2-5 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CD146/MCAM Antibody IHC Application Image
CD146 / MCAM Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunohistochemical staining of formalin fixed, paraffin-embedded human lymphonode showing membrane staining of endothelial cells (red arrow) (0.2 μg/mL).
CD146 / MCAM Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunohistochemical staining of formalin fixed, paraffin-embedded human stomach showing (The left panel: 2 μg/mL. The right panel: 0.2 μg/mL). 1. Membrane staining of endothelial cells (red arrow) 2. Smooth muscle cells (black arrow) 3. Epithelial cells (green arrow)
Other CD146/MCAM Antibody Products
Reactivity: Human  
Application: ELISA  
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      10115-R012-50
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      Application: FCM  IF  ICC/IF  
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      10115-MM01-A-50
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      10115-R044-A-100
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      Reactivity: Human  
      Application: FCM  IF  ICC/IF  
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      Reactivity: Mouse  
      Application: ELISA  
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        Reactivity: Mouse  
        Application: ELISA  
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          50794-RP01-100
           
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          Application: ELISA  WB  IHC-P  IP  
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          200 µg 
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          CD146/MCAM Background

          The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.

          Human CD146/MCAM References
        • Mills L, et al. (2002) Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 62(17): 5106-14.
        • Taira E, et al. (2004) Characterization of Gicerin/MUC18/CD146 in the rat nervous system. J Cell Physiol. 198(3): 377-87.
        • Fritzsche FR, et al. (2008) CD146 protein in prostate cancer: revisited with two different antibodies. Pathology. 40(5): 457-64.
        • Bidlingmaier S, et al. (2009) Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 69(4): 1570-7.
        • Boneberg EM, et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 78(3): 325-31.
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          Catalog: 10115-RP02-200
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          All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
          Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"