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CD146 / MCAM Antibody (PE), Mouse MAb

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Human CD146/MCAM Antibody Product Information
Immunogen:Recombinant Human CD146 / MCAM protein (Catalog#10115-H08H)
Clone ID:01
Ig Type:Mouse IgG1
Concentration:10 μl/Test, 0.1 mg/ml
Formulation:Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD146 / MCAM (rh CD146 / MCAM; Catalog#10115-H08H; NP_006491.2; Met 1-Gly 559) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Human CD146/MCAM Antibody Usage Guide
Specificity:Human CD146 / MCAM
No cross-reactivity in ELISA with
Human CD66a
Human CD166
Human CD106
Human CD226
Human BCAM
Human cell lysate (293 cell line)
Application:FCM
Storage:This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Human CD146/MCAM Antibody FC Application Image
CD146 / MCAM Antibody (PE), Mouse MAb, Flow cytometric
[Click to enlarge image]
Caption:
Profile of anti-CD146 reactivity on HeLa cells analyzed by flow cytometry.
Other CD146/MCAM Antibody Products
Reactivity: Human  
Application: ELISA  
    10115-RP01-400
    10115-RP01-100
    10115-RP01-200
     
    100 µg 
    200 µg 
    Add to Cart
    Reactivity: Human  
    Application: ELISA  IHC-P  
    • Slide 1
    • Slide 1
    10115-RP02-200
    10115-RP02-50
    10115-RP02-100
    200 µg 
    50 µg 
    100 µg 
    Add to Cart
    Reactivity: Human  
    Application: ELISA  
      10115-MM04-200
      10115-MM04-50
      10115-MM04-100
      200 µg 
      50 µg 
      100 µg 
      Add to Cart
      Reactivity: Human  
      Application: ELISA  IP  
      • Slide 1
      10115-MM05-200
      10115-MM05-50
      10115-MM05-100
      200 µg 
      50 µg 
      100 µg 
      Add to Cart
      Reactivity: Human  
      Application: ELISA  IHC-P  FCM  IF  ICC/IF  
      • Slide 1
      • Slide 1
      • Slide 1
      • Slide 1
      10115-R044-50
      10115-R044-100
      50 µg 
      100 µg 
      Add to Cart
      Reactivity: Human  
      Application: IHC-P  
      • Slide 1
      • Slide 1
      • Slide 1
      10115-R005-50
      10115-R005-100
      50 µg 
      100 µg 
      Add to Cart
      Reactivity: Human  
      Application: ELISA  WB  IHC-P  
      • Slide 1
      • Slide 1
      • Slide 1
      • Slide 1
      10115-R012-50
      10115-R012-100
      50 µg 
      100 µg 
      Add to Cart
      Reactivity: Human  
      Application: FCM  IF  ICC/IF  
      • Slide 1
      • Slide 1
      10115-R044-F-50
      10115-R044-F-100
      50 Tests 
      100 Tests 
      Add to Cart
      Reactivity: Human  
      Application: FCM  
      • Slide 1
      10115-MM01-200
      10115-MM01-100
      10115-MM01-50
      200 µg 
      100 µg 
      50 µg 
      Add to Cart
      Reactivity: Human  
      Application: FCM  
      • Slide 1
      10115-MM01-A-50
      10115-MM01-A-100
      50 Tests 
      100 Tests 
      Add to Cart
      Reactivity: Human  
      Application: FCM  
      • Slide 1
      10115-R044-A-100
      10115-R044-A-50
      100 Tests 
      50 Tests 
      Add to Cart
      Reactivity: Human  
      Application: FCM  IF  ICC/IF  
      • Slide 1
      • Slide 1
      10115-R044-P-50
      10115-R044-P-100
      50 Tests 
      100 Tests 
      Add to Cart
      Reactivity: Mouse  
      Application: ELISA  
        50794-R112-100
        50794-R112-50
        100 µg 
        50 µg 
        Add to Cart
        Reactivity: Mouse  
        Application: ELISA  
          50794-RP01-400
          50794-RP01-200
          50794-RP01-100
           
          200 µg 
          100 µg 
          Add to Cart
          Reactivity: Mouse  
          Application: ELISA  WB  IHC-P  IP  
          • Slide 1
          • Slide 1
          • Slide 1
          • Slide 1
          50794-RP02-200
          50794-RP02-100
          50794-RP02-50
          200 µg 
          100 µg 
          50 µg 
          Add to Cart
          CD146/MCAM Background

          The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.

          Human CD146/MCAM References
        • Mills L, et al. (2002) Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 62(17): 5106-14.
        • Taira E, et al. (2004) Characterization of Gicerin/MUC18/CD146 in the rat nervous system. J Cell Physiol. 198(3): 377-87.
        • Fritzsche FR, et al. (2008) CD146 protein in prostate cancer: revisited with two different antibodies. Pathology. 40(5): 457-64.
        • Bidlingmaier S, et al. (2009) Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 69(4): 1570-7.
        • Boneberg EM, et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 78(3): 325-31.
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          Catalog: 10115-MM01-P-50
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          All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
          Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"