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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Cynomolgus monkey CD14 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||CG90174-G-F|
|Cynomolgus monkey CD14 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||CG90174-G-H|
|Cynomolgus monkey CD14 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||CG90174-G-M|
|Cynomolgus monkey CD14 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||CG90174-G-N|
|Cynomolgus monkey CD14 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||CG90174-G-Y|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns.