Rat CD14 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80308-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD14 cDNA Clone Datasheet

CD14 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_021744.1
cDNA Size:	1119
Gene Synonym:	Cd14
Source:	Rat

CD14 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus CD14 molecule DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 801 T/A and 1084 T/C not causing the amino acid variation.

CD14 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD14 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD14 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGCTTA TGCTCGGCTT GTTGCTGTTG CCTTTGACAC TGGTGCATGC CTCTCCCGCC
61       ACACCAGAAC CCTGTGAGCT GGACCAGGAC GAGGAAAGTG TCCGCTGCTA CTGCAACTTC
121      TCAGATCCGC AGCCCAATTG GTCCAGCGCT TTTCTATGCG CGGGGGCGGA AGACGTGGAG
181      TTCTACGGCG GCGGTCGCAG CCTGGAGTAC CTTCTAAAGC GTGTGGACAC GGAAGCAAAT
241      CTGGGGCAGT ACACTGATAT TATCAGGTCT CTGCCCTTGA AGAGGCTTAC TGTGCGGTCC
301      GCGCGGGTTC CTACTCAGAT TCTATTCGGA ACCCTGCGTG TGCTCGGATA TTCTGGCCTC
361      CGGGAACTGA CTCTTGAAAA CCTCGAGGTA ACCGGCACCG CGCTTTCGCC GCTTCTGGAT
421      GCCACCGGAC CCGATCTCAA CACCTTGAGC CTCCGCAACG TGTCGTGGGC AACAACGGAT
481      ACCTGGCTCG CAGAACTGCA GCAGTGGCTA AAGCCTGGAC TCAAGGTACT AAGTATTGCC
541      CAAGCACACT CACTCAACTT TTCTTGCAAA CAGGTCGGCG TCTTCCCTGC CCTTGCCACC
601      TTAGATCTGT CTGACAACCC TGAGTTGGGC GAGAAAGGAC TGATCTCGGC CCTCTGTCCG
661      CACAAATTCC CGACCCTCCA AGTTTTAGCG CTGCGCAACG CGGGGATGGA GACGACCAGC
721      GGCGTGTGCT CTGCGCTGGC CGCAGCAAGA GTGCCACTGC AGGCACTGGA TCTCAGTCAC
781      AATTCACTGC GGGATACTGC AGGCACTCCG AGCTGTGACT GGCCCAGTCA GCTAAACTCG
841      CTCAATCTAT CTTTCACTGG GCTGGAGCAC GTACCTAAAG GGCTGCCAGC CAAGCTTAGC
901      GTGCTTGATC TCAGTTACAA CAGGCTGGAT AGGAAACCTA GGCCAGAGGA GCTGCCTGAA
961      GTGGGGAGCC TGTCACTTAC AGGAAATCCC TTTTTGCACT CTGAATCCCA GTCGGAGGCG
1021     TATAACTCTG GCGTAGTCAT AGCCACAGCT CTTTCACCCG GATCAGCGGG CTTGTCAGGA
1081     ACTCTGGCTT TGCTCCTAGG ACATCGCCTC TTTGTTTAA

Quality Control:cDNA sequencing results is available upon request.