Anti-CD14 Antibody

Cat: 50023-R002
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Anti-CD14 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-CD14 Antibody
Validated applications
WB,ELISA,ELISA(Det)
Species reactivity
Reacts with: Mouse
Specificity
Mouse CD14
Immunogen
Recombinant Mouse CD14 protein (Catalog#50023-M03H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD14 extracellular domain (rM CD14; Catalog#50023-M03H; NP_033971.1; Met 1-Pro 345).
Source
Monoclonal Rabbit IgG Clone #2
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD14 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
ELISA(Det) 1:5000-1:50000 This antibody will detect Mouse CD14 in ELISA pair set (Catalog: # SEK50023). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 50023-R217).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD14 Antibody (Rabbit Monoclonal antibody) Images
Mouse CD14 Western blot (WB) 7024

Anti-CD14 rabbit monoclonal antibody at 1:500 dilution

Lane A: SW480 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:40 kDa

Observed band size:63 kDa

Anti-CD14 Antibody Alternative Names
Anti-CD14 Antibody
CD14 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns.

Full Name
CD14 molecule
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • SD Wright, et al. (1990) CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 249 (4975): 1431-3.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD14 Antibody Rabbit Polyclonal ELISA,IHC-P 90174-T24
    Anti-CD14 Antibody Mouse Monoclonal FCM 90174-MM10
    Anti-CD14 Antibody Rabbit Polyclonal ELISA 90174-RP01
    Anti-CD14 Antibody (FITC) Mouse Monoclonal FCM 90174-MM12-F
    Anti-CD14 Antibody (FITC) Mouse Monoclonal FCM 90174-MM10-F
    Anti-CD14 Antibody Rabbit Polyclonal ELISA 90174-T16
    Anti-CD14 Antibody Mouse Monoclonal FCM 90174-MM12
    Anti-CD14 Antibody Mouse Monoclonal IHC-P 90174-MM19
    Anti-CD14 Antibody Rabbit Polyclonal ELISA 80308-RP01
    Anti-CD14 Antibody Rabbit Polyclonal ELISA 80308-T16
    Anti-CD14 Antibody (APC) Rabbit Monoclonal FCM 50023-R503-A
    Anti-CD14 Antibody Rabbit Monoclonal FCM,ICC/IF,IF 50023-R503
    Anti-CD14 Antibody (FITC) Rabbit Monoclonal FCM 50023-R503-F
    Anti-CD14 Antibody Rabbit Polyclonal ELISA 50023-T16
    Anti-CD14 Antibody (PE) Rabbit Monoclonal FCM 50023-R503-P
    Anti-CD14 Antibody (PerCP) Rabbit Monoclonal FCM 50023-R503-C
    Anti-CD14 Antibody Rabbit Polyclonal ELISA 50023-RP01
    Anti-CD14 Antibody Rabbit Polyclonal ELISA 50023-RP02
    Anti-CD14 Antibody Rabbit Monoclonal ELISA(Cap) 50023-R217
    Anti-CD14 Antibody (PerCP) Rabbit Monoclonal FCM 10073-R002-C
    Anti-CD14 Antibody Rabbit Polyclonal ELISA 10073-RP01
    Anti-CD14 Antibody Rabbit Monoclonal IHC-P 10073-R001
    Anti-CD14 Antibody (APC) Mouse Monoclonal FCM 10073-MM06-A
    Anti-CD14 Antibody Mouse Monoclonal FCM 10073-MM06
    Anti-CD14 Antibody (PE) Rabbit Monoclonal FCM 10073-R002-P
    Anti-CD14 Antibody (FITC) Rabbit Monoclonal FCM 10073-R002-F
    Anti-CD14 Antibody (APC) Rabbit Monoclonal FCM 10073-R002-A
    Anti-CD14 Antibody Mouse Monoclonal ELISA 10073-MM01
    Anti-CD14 Antibody (PE) Mouse Monoclonal FCM 10073-MM06-P
    Anti-CD14 Antibody (FITC) Mouse Monoclonal FCM 10073-MM06-F
    Anti-CD14 Antibody Rabbit Monoclonal FCM 10073-R002
    Anti-CD14 Antibody Rabbit Monoclonal ELISA 10073-R107
    Anti-CD14 Antibody Rabbit Polyclonal ELISA,IHC-P 10073-RP02
    Anti-CD14 Antibody Mouse Monoclonal IHC-P 10073-MM17
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