CD132 / IL2RG Antibody (Cytokine)

CD132 / IL2RG Antibody Datasheet

CD132 / IL2RG Antibody Product Information

• Immunogen: Recombinant Mouse CD132 / IL2RG protein (Catalog#50087-M08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse CD132 / IL2RG (rM CD132 / IL2RG; Catalog#50087-M08H; NP_038591.1; Met 1-Ala 263). Total IgG was purified by Protein A affinity chromatography.

CD132 / IL2RG Antibody Usage Guide

• Specificity: Mouse CD132 / IL2RG
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse IL2RG in WB.
• Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Mouse IL2RG. The detection limit for Mouse IL2RG is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD132 / IL2RG Antibody Background

The interleukin 2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, which binds and responds to the cytokine IL-2. Three distinct chains of IL-2R, termed as α, β and γ, which are non-covalently associated are identified. The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ chain, along with the β subunit which are members of the type I cytokine receptor family. The α chain of the IL-2R can bind to the β chain before receptor interaction with IL-2. The γ chain alone has a very weak affinity for IL-2, but after the ligand is bound to the α/β heterodimer, the γ chain becomes recruited to the complex to form a very stable macromolecular quaternary ligand/receptor complex. It is proposed that the γ chain is common to the IL2, IL4, IL7, IL21 and probably also the IL13 receptors.

References

1. Robb RJ. et al., 1981, J Exp Med. 154 (5): 1455-1474.
2. Leonard WJ. et al., 1982, Nature. 300 (5889): 267-269.
3. Sharon M. et al., 1986, Science. 234 (4778): 859-863.
4. Tsudo M. et al., 1987, Proc Natl Acad Sci. 84 (12): 4215-4218.
5. Takeshita T. et al., 1992, J Immunol. 148 (7): 2154-2158.
6. Johnson K. et al., 1994, Eur Cytokine Netw. 5 (1): 23-34.