CD131 / CSF2RB / IL3RB / IL5RB Antibody

CD131 / CSF2RB / IL3RB / IL5RB Antibody Datasheet

CD131 / CSF2RB / IL3RB / IL5RB Antibody Product Information

• Immunogen: Recombinant Human CSF2RB protein (Catalog#10516-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 5C7B1
• Ig Type: Mouse IgG2a
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD131 / CSF2RB / IL3RB / IL5RB (rh CD131 / CSF2RB / IL3RB / IL5RB; Catalog#10516-H08H; NP_000386.1; Met 1-Trp 443). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD131 / CSF2RB / IL3RB / IL5RB Antibody Usage Guide

• Specificity: Human CD131 / CSF2RB / IL3RB / IL5RB
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CSF2Rb in WB. Using a DAB detection system, the detection limit for Human CSF2Rb is approximately 2 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CSF2Rb. The detection limit for Human CSF2Rb is approximately 0.0195 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD131 / CSF2RB / IL3RB / IL5RB Antibody Background

Colony stimulating factor 2 receptor, beta, low-affinity, also known as Cytokine receptor common subunit beta, CSF2RB, CDw131, and CD131, is a single-pass type I membrane protein which belongs to thetype I cytokine receptor family and Type 4 subfamily. It is a 897 amino acid protein which the sequence is further processed into a mature form. The cytokine receptor is a heterodimer of an alpha and a beta subunit, and this beta subunit (CD131) is also the common and high affinity receptor to the interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor. It contains two fibronectin type-III domains. The WSXWS motif of CD131 appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif of CD131 is required for JAK interaction and/or activation.

References

1. Mulhern, TD. et al., 2000, J. Mol. Biol. 297: 989-1001.
2. Carr, PD. et al., 2001, Cell. 104: 291-300.
3. Carr, PD. et al., 2006, Acta Crystallogr. 62: 509-513.
4. Selleri, S. et al., 2008,J Transl Med. 6: 17.