CD13 / ANPEP Antibody

CD13 / ANPEP Antibody Datasheet

CD13 / ANPEP Antibody Product Information

• Immunogen: Recombinant Human CD13 / ANPEP protein (Catalog#10051-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 122
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD13 / ANPEP (rh CD13 / ANPEP; Catalog#10051-H08H; NP_001141.2; Lys 69-Lys 967, 603 Met/Ile).

CD13 / ANPEP Antibody Usage Guide

• Specificity: Human CD13 / ANPEP
• Immunochemistry: IHC-P: 1-10 μg/mL

  

  Immunochemical staining of human ANPEP in human liver with rabbit monoclonal antibody (5 μg/mL, formalin-fixed paraffin embedded sections).

  Immunochemical staining of human ANPEP in human renal carcinoma with rabbit monoclonal antibody (5 μg/mL, formalin-fixed paraffin embedded sections).

• Western blot: WB: 1-2 μg/mL

  A B Sample (whole cell lysate) THP1 U937 Sample Volume (μg/lane) 30 30 Gel 13% SDS-PAGE reducing gel Recommended Concentration 1-2 μg/ml Secondary Antibody Dylight 800-labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution.

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ANPEP.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD13 / ANPEP Antibody Background

aminopeptidase N (APN, ANPEP), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. CD13/ANPEP plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/ANPEP is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas. Furthermore, human CD13/ANPEP is a receptor for one strain of human coronavirus strain HCV-229E that is an important cause of upper respiratory tract infections.

References

1. Falk, K. et al., 1994, Immuno genetics. 39: 230-242.
2. Petrovic, N. et al., 2003, J. Biol. Chem. 278: 49358-49368.
3. Yeager, C.L. et al., 1992, Nature. 357: 420-422.
4. Bhagwat, S.V. et al., 2003, Blood. 101: 1818-1826.
5. Pasqualini, R. et al., 2000, Cancer Res. 60: 722-727.