Anti-CD13 Antibody

Cat: 10051-RP02
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Anti-CD13 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CD13 Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human CD13
Immunogen
Recombinant Human ANPEP protein (Catalog#10051-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human ANPEP (rh ANPEP; Catalog#10051-H08H; NP_001141.2; Lys 69-Lys 967). ANPEP specific IgG was purified by human ANPEP affinity chromatography .
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD13 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  
IHC-P 1:2500-1:10000  
IP 0.5-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD13 Antibody (Rabbit Polyclonal antibody) Images
Human CD13 / ANPEP Immunohistochemistry(IHC) 4700
Immunochemical staining of human ANPEP in human renal carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Human CD13 / ANPEP Immunohistochemistry(IHC) 4702
Immunochemical staining of human ANPEP in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Human CD13 / ANPEP Western blot (WB) 13093

Anti-ANPEP rabbit polyclonal antibody at 1:500 dilution

Lane A: U937 Whole Cell Lysate

Lane B: THP-1 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:110 kDa

Observed band size:130 kDa

Human CD13 / ANPEP Immunoprecipitation(IP) 17661

ANPEP was immunoprecipitated using:

Lane A:0.5 mg THP1 Whole Cell Lysate

2 µL anti-ANPEP rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ANPEP rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 110 kDa

Observed band size: 110 kDa

Anti-CD13 Antibody Alternative Names
Anti-APN Antibody;Anti-CD13 Antibody;Anti-GP150 Antibody;Anti-LAP1 Antibody;Anti-P150 Antibody;Anti-PEPN Antibody
CD13 Background Information

Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.

Full Name
alanyl (membrane) aminopeptidase
References
  • Pasqualini R, et al. (2000) Aminopeptidase N is a receptor for tumor-homing peptides and a target for inhibiting angiogenesis.Cancer Res. 60(3): 722-7.
  • Mabjeesh SJ, et al. (2005) Aminopeptidase N gene expression and abundance in caprine mammary gland is influenced by circulating plasma peptide. J Dairy Sci. 88(6): 2055-64.
  • Dunkel B, et al. (2009) Neutrophil and platelet activation in equine recurrent airway obstruction is associated with increased neutrophil CD13 expression, but not platelet CD41/61 and CD62P or neutrophil-platelet aggregate formation. Vet Immunol Immunopathol. 131(1-2): 25-32.
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