>Rabbit PAb Antibody
>CD127 / IL-7RA Antibody (Antigen Affinity Purified)
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CD127 / IL-7RA Antibody ( Antigen Affinity Purified ) Datasheet
|Order or Inquire for CD127 / IL-7RA Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 0.5 ng/lane in WB|
|Detection limit is 0.00245 ng/well in ELISA|
CD127 / IL-7RA Antibody Product Information
Recombinant Mouse CD127 / IL-7RA protein (Catalog#50090-M08H)
|Antibody Type :||Rabbit Polyclonal Antibody ( Antibody Purification Platform )|
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS with 5% trehalose|
Produced in rabbits immunized with purified, recombinant Mouse CD127 / IL-7RA (rM CD127 / IL-7RA; Catalog#50090-M08H; NP_032398.3; Met 1-Asp 239). CD127 / IL-7RA specific IgG was purified by Mouse CD127 / IL-7RA affinity chromatography.
CD127 / IL-7RA Antibody Usage Guide
Mouse CD127 / IL-7RA
|Western blot :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse IL7R in WB. Using a DAB detection system, the detection limit for Mouse IL7R is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.|
|Direct ELISA :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse IL7R. The detection limit for Mouse IL7R is approximately 0.00245 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
CD127 / IL-7RA Antibody Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|CD127/IL-7RA||Human||CD127/IL-7RA/Fc Protein, Recombinant||10975-H03H|
|CD127/IL-7RA||Human||CD127/IL-7RA Protein, Recombinant||10975-H08H|
|CD127/IL-7RA||Mouse||CD127/IL-7RA Protein, Recombinant||50090-M08H|
|CD127/IL-7RA||Rat||IL7R / IL7RA Protein, Recombinant||80083-R05H|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|WB, ELISA||Mouse Monoclonal Antibody||10975-MM12|
|WB, ELISA||CD127/IL-7RA Antibody, Rabbit PAb||10975-RP01|
|WB, ELISA||CD127/IL-7RA Antibody, Rabbit PAb (Antigen Affinity Purified)||10975-RP02|
|WB, ELISA||CD127/IL-7RA Antibody, Rabbit PAb (Antigen Affinity Purified)||50090-RP02|
CD127 / IL-7RA Antibody Background
Interleukin 7 Receptor alpha (IL-7Rα), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7Rα is majorly expressed on T cells and their precursors, and early in B cell development as well, prior to the appearance of surface IgM. Mature human IL-7Rα consists of a 219 aa extracellular domain (ECD) with one fibronectin type-III domain and a WSxWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain. Within the ECD, human IL-7Rα shares 67% aa sequence identity with mouse IL-7Rα. Mutations in the human IL-7Rα gene cause a type of severe combined immunodeficiency in which the major deficiencies are in T cell development, whereas B and NK cells are relatively normal in number. Therefore, the IL-7Rα is critically required for the proper development and function of lymphoid cells. In addition to IL-7, IL-7Rα also associates with TSLPR to form the functional receptor for thymic stromal lymphopoietin (TSLP) which indirectly regulates T cell development by modulating dendritic cell activation. Dynamic regulation of IL-7Rα is important for the generation of appropriate immune responses.
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