CD127 / IL-7RA Antibody (Antigen Affinity Purified) (Cytokine)

CD127 / IL-7RA Antibody ( Antigen Affinity Purified ) Datasheet

CD127 / IL-7RA Antibody Product Information

• Immunogen: Recombinant Mouse CD127 / IL-7RA protein (Catalog#50090-M08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse CD127 / IL-7RA (rM CD127 / IL-7RA; Catalog#50090-M08H; NP_032398.3; Met 1-Asp 239). CD127 / IL-7RA specific IgG was purified by Mouse CD127 / IL-7RA affinity chromatography.

CD127 / IL-7RA Antibody Usage Guide

• Specificity: Mouse CD127 / IL-7RA
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse IL7R in WB. Using a DAB detection system, the detection limit for Mouse IL7R is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse IL7R. The detection limit for Mouse IL7R is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD127 / IL-7RA Antibody Background

Interleukin 7 Receptor alpha (IL-7Rα), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7Rα is majorly expressed on T cells and their precursors, and early in B cell development as well, prior to the appearance of surface IgM. Mature human IL-7Rα consists of a 219 aa extracellular domain (ECD) with one fibronectin type-III domain and a WSxWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain. Within the ECD, human IL-7Rα shares 67% aa sequence identity with mouse IL-7Rα. Mutations in the human IL-7Rα gene cause a type of severe combined immunodeficiency in which the major deficiencies are in T cell development, whereas B and NK cells are relatively normal in number. Therefore, the IL-7Rα is critically required for the proper development and function of lymphoid cells. In addition to IL-7, IL-7Rα also associates with TSLPR to form the functional receptor for thymic stromal lymphopoietin (TSLP) which indirectly regulates T cell development by modulating dendritic cell activation. Dynamic regulation of IL-7Rα is important for the generation of appropriate immune responses.

References

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