Mouse IL3Ra Gene cDNA Clone / ORF Clone

IL3RA cDNA Clone Datasheet

IL3RA cDNA Clone Product Information

• Gene Bank Ref.ID: NM_008369.1
• cDNA Size: 1191
• Gene Synonym: CD123; SUT-1; CDw123
• Source: Mouse

IL3RA cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus interleukin 3 receptor, alpha chain DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for four point mutations: 338 A/G resulting in the amino acid asp substitution by gly, 367 C/A resulting in the amino acid leu substitution by met, 882 T/C and 1020 G/T not causing the amino acid variation.

IL3RA cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL3RA cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL3RA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCGCCA ACCTGTGGCT CATCCTGGGG CTCCTGGCAT CCCACTCTTC AGATCTGGCT
61       GCGGTCCGTG AGGCTCCACC CACTGCTGTG ACCACGCCCA TCCAGAACCT GCATATCGAC
121      CCCGCCCACT ACACACTGAG CTGGGACCCC GCCCCCGGCG CTGACATCAC GACAGGGGCT
181      TTTTGCAGGA AGGGCAGGGA CATCTTTGTC TGGGCTGACC CCGGGCTTGC CCGCTGCTCA
241      TTCCAGTCGC TCTCCCTCTG CCATGTGACC AACTTCACCG TCTTCCTGGG GAAGGACAGG
301      GCTGTGGCAG GATCCATCCA GTTCCCCCCA GATGATGGTG GCGACCACGA GGCCGCAGCC
361      CAGGACATGC GCTGCTGGGT GCATGAGGGG CAGCTGAGCT GCCAATGGGA GCGGGGTCCG
421      AAAGCAACGG GTGACGTGCA CTACCGGATG TTCTGGCGTG ATGTGCGGCT CGGTCCTGCC
481      CACAACCGCG AGTGTCCACA CTACCACAGC CTTGATGTCA ACACCGCTGG CCCCGCCCCT
541      CATGGTGGCC ATGAAGGCTG CACCCTTGAC CTCGACACTG TCCTTGGCTC CACCCCCAAC
601      AGCCCTGACC TTGTCCCCCA GGTCACCATC ACCGTCAACG GCAGTGGCCG CGCTGGCCCC
661      GTGCCCTGCA TGGACAACAC TGTGGACCTG CAGCGCGCAG AGGTCCTGGC CCCGCCCACG
721      TTGACAGTGG AGTGCAACGG CTCTGAGGCC CATGCCCGAT GGGTCGCGCG GAACAGATTC
781      CACCATGGCC TCCTTGGGTA CACCCTGCAG GTCAACCAGA GCTCCCGCTC AGAGCCACAG
841      GAATACAATG TCTCCATCCC CCACTTCTGG GTCCCCAACG CCGGTGCCAT CTCCTTCCGG
901      GTCAAGTCGA GGTCAGAGGT TTACCCTCGG AAGCTCAGCA GCTGGAGCGA AGCCTGGGGC
961      CTCGTCTGCC CCCCAGAGGT GATGCCTGTG AAGACAGCCT TGGTGACTTC AGTGGCTACT
1021     GTGCTGGGGG CAGGGCTCGT GGCAGCTGGG CTCCTGCTGT GGTGGAGGAA GTCGCTGCTC
1081     TACCGCCTGT GCCCACCCAT TCCACGCCTG CGCCTGCCCT TGGCAGGGGA GATGGTCGTG
1141     TGGGAACCAG CTCTTGAAGA CTGCGAGGTG ACGCCTGTGA CAGACGCCTG A

 

Quality Control:cDNA sequencing results is available upon request.