IL3RA / CD123 Protein

Interleukin-3 Receptor Subunit Alpha ( IL3RA / CD123 ) Protein

IL3RA / CD123 Protein Product Information

• Synonym: IL3RA,RP11-261P4.2, CD123, IL3R, IL3RAY, IL3RX, IL3RY, MGC34174, hIL-3Ra
• Protein Construction: A DNA sequence encoding the human IL3RA ( NP_002174.1 ) extracellular domain ( Met 1 - Arg 305 ) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus
• Source: Human
• Expression Host: Human Cells

IL3RA / CD123 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA
  Immobilized human IL3 at 20 μg/ml ( 100 μl/well ) can bind human IL3RA with a linear ranger of 0.064 - 1.6 μg/ml
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Thr 19
• Molecular Mass: The recombinant human IL3RA / Fc is a disulfide-linked homodimer. The reduced monomer comprises 535 amino acids and has a predicted molecular mass of 61 kDa. As a result of glycosylation, the apparent molecular mass of rh IL3RA / Fc monomer is approximately 80-90 kDa in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL3RA protein

IL3RA / CD123 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL3RA / CD123 Protein Description

Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I  membrane protein which belongs to the type I  cytokine receptor family and Type 5 subfamily. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and / or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity.

References

1. Muñoz, L. et al., 2001, Haematologica. 86 (12): 1261-9.
2. Moretti, S. et al., 2001, J Biol Regul Homeost Agents 15 (1): 98-100.
3. Del Giudice, I. et al., 2004, Haematologica. 89 (3): 303-8.
4. Du, X. et al., 2007, J Immunother. 30 (6): 607-13.
5. Djokic, M. et al., 2009, Haematologica. 94 (7): 1016-9.