Anti-IL3RA Antibody

Cat: 10518-MM07
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Anti-IL3RA Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-IL3RA Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human IL3RA
No cross-reactivity in ELISA with Human IL6R Human IL2Rb Human cell lysate (293 cell line)
Immunogen
Recombinant Human IL3RA / CD123 protein (Catalog#10518-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD123 / IL3RA (rh CD123 / IL3RA; Catalog#10518-H08H; NP_002174.1; Met 1-Arg 305). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #6H2E3F3
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-IL3RA Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-IL3RA Antibody Alternative Names
Anti-CD123 Antibody;Anti-hIL-3Ra Antibody;Anti-IL3R Antibody;Anti-IL3RAY Antibody;Anti-IL3RX Antibody;Anti-IL3RY Antibody
IL3RA Background Information

Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily. The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and / or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recent studies have shown that interleukin-3 receptor alpha (CD123) is highly expressed on leukemia stem cells of patients with acute myeloid leukemia, and is correlated with tumor load and poor prognosis. CD123 was highly expressed in the bone marrow of the patients with myelodysplastic syndrome (MDS), significantly correlated with the proportion of bone marrow blasts, and thus might be the marker of MDS malignant clone. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity.

Full Name
interleukin 3 receptor, alpha (low affinity)
References
  • Del Giudice I, et al. (2004) The diagnostic value of CD123 in B-cell disorders with hairy or villous lymphocytes. Haematologica. 89(3): 303-8.
  • Du X, et al. (2007) New immunotoxins targeting CD123, a stem cell antigen on acute myeloid leukemia cells. J Immunother. 30(6): 607-13.
  • Yue LZ, et al. (2010) Expression of CD123 and CD114 on the bone marrow cells of patients with myelodysplastic syndrome. Chin Med J (Engl). 123(15): 2034-2037.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-IL3RA Antibody Rabbit Monoclonal WB,ELISA,IP 10518-R017
    Anti-IL3RA Antibody Rabbit Monoclonal ELISA,ELISA(Det) 10518-R012
    Anti-IL3RA Antibody Rabbit Polyclonal ELISA 10518-RP02
    Anti-IL3RA Antibody Mouse Monoclonal ELISA(Cap) 10518-MM01
    Anti-IL3RA Antibody Rabbit Polyclonal ELISA 10518-RP01
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