CD123 / IL3RA Antibody (Antigen Affinity Purified) (Cytokine)

IL3RA / CD123 Antibody (Antigen Affinity Purified) Datasheet

IL3RA / CD123 Antibody Product Information

• Immunogen: Recombinant Human IL3RA / CD123 protein (Catalog#10518-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant Human IL3RA / CD123 (rh IL3RA / CD123; Catalog#10518-H08H; NP_002174.1; Met 1-Arg 305). IL3RA / CD123 specific IgG was purified by Human IL3RA / CD123 affinity chromatography.

IL3RA / CD123 Antibody Usage Guide

• Specificity: Human IL3RA / CD123
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human IL3RA in WB. Using a DAB detection system, the detection limit for Human IL3RA is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human IL3RA. The detection limit for Human IL3RA is approximately 0.0049 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

IL3RA / CD123 Antibody Background

Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I  membrane protein which belongs to the type I  cytokine receptor family and Type 5 subfamily. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and / or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity.

References

1. Muñoz, L. et al., 2001, Haematologica. 86 (12): 1261-9.
2. Moretti, S. et al., 2001, J Biol Regul Homeost Agents 15 (1): 98-100.
3. Del Giudice, I. et al., 2004, Haematologica. 89 (3): 303-8.
4. Du, X. et al., 2007, J Immunother. 30 (6): 607-13.
5. Djokic, M. et al., 2009, Haematologica. 94 (7): 1016-9.