Mouse CD112 / PVRL2 Protein (His Tag)

Poliovirus Receptor-Related 2 ( CD112 / Nectin2 ) Protein Datasheet

CD112 / Nectin2 Protein Product Information

• Synonym: Pvrl2 , AI325026, AI987993, Cd112, MPH, Pvr, Pvs, nectin-2
• Protein Construction: A DNA sequence encoding the mouse PVRL2 (NP_033016.3) extracellular domain (Met 1-Gly 351) was fused with a polyhistidine tag at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

CD112 / Nectin2 Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA.
  1. Immobilized mouse CD112-His (Cat: 50318-M08H) at 10 μg/ml (100 μl/well) can bind biotinylated mouse DNAM1-His (Cat: 50232-M08H) with a linear range of 0.156-5.0 μg/ml.
  2. Immobilized mouse CD112-His (Cat: 50318-M08H) at 10 μg/ml (100 μl/well) can bind mouse DNAM1-Fc (Cat: 50232-M03H) with a linear range of 0.03-1.0 μg/ml.
• Endotoxin: < 1.0 EU per 1μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 32
• Molecular Mass: The secreted recombinant mouse PVRL2 comprises 331 amino acids with a predicted molecular mass of 36 kDa. As a result of glycosylation, it migrates as an approximately 40-45 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

• SDS-PAGE:

CD112 / Nectin2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD112 / Nectin2 Protein Description

Poliovirus receptor-related 2 (PVRL2), also known as Nectin-2 and CD112, is a single-pass type I  transmembrane glycoprotein belonging to the nectin family, and structurally comprises two Ig-like C2-type domains and one Ig-like V-type domain in the extracellular region. Mouse CD112 is a 70 to 78 kDa type I  TM glycoprotein that is found on a variety of cell types. As a homophilic adhesion molecule, CD112 is widely expressed in human tissues including hematopoietic cells and mediates intercellular junctions of adjacent cells. CD112 has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 can induce NK cell- and CD8+T cell-mediated cytotoxicity and cytokine secretion. CD112 protein serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and is involved in cell to cell spreading of these viruses. Disruption of both alleles of the murine CD112 gene resulted in morphologically aberrant spermatozoa. CD112 has also been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy. Mouse CD112 ECD shares 72%, 77%, 95% aa identity with the ECD in human, canine and rat CD112, respectively.

References

1. Lopez, M. et al., 1998, Blood. 92: 4602-4611.
2. Cocchi, F. et al., 2000, J. Virol. 74: 3909-3917.
3. Peng, YF. et al., 2002, Oncogene. 21: 4108-4119.
4. Tahara HS. et al., 2005, Biochem Biophys Res Commun. 329: 996-1000.
5. Bachelet, I.. et al., 2006, J. Biol. Chem. 281: 27190-27196.
6. Wang, L. et al., 2009, Vet Immunol Immunopathol.