Nectin-2 Matched ELISA Antibody Pair Set,Human

Cat: SEK10005
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Nectin-2 Matched ELISA Antibody Pair Set,Human General Information
Product name
Nectin-2 Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human Nectin-2
Assay range
15.625-1000
Materials provided
1. Capture Antibody 0.25 mg/mL of rabbit anti-CD112 monoclonal antibody, Dilute to a working concentration of 2.0 μg/mL in CBS before coating. (Catalog: # 10005-R005)
2. Detection Antibody 0.5 mg/mL rabbit anti-CD112 polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.1 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 50 ng of recombinant CD112. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
Product overview
This Nectin-2 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human Nectin-2 . It contains Human Nectin-2 capture antibody, Human Nectin-2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Nectin-2 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Nectin-2 Matched ELISA Antibody Pair Set,Human Images
Human CD112/Nectin-2/PVRL2 ELISA Pair Set Standard Curve 13773
Nectin-2 Matched ELISA Antibody Pair Set,Human Alternative Names
CD112 Matched ELISA Antibody Pair Set, Human;HVEB Matched ELISA Antibody Pair Set, Human;Nectin-2 Matched ELISA Antibody Pair Set, Human;PRR2 Matched ELISA Antibody Pair Set, Human;PVRR2 Matched ELISA Antibody Pair Set, Human
Nectin-2 Background Information

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.

Full Name
poliovirus receptor-related 2 (herpesvirus entry mediator B)
References
  • Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.
  • Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    Nectin-2 ELISA Kit, Human Serum KIT10005
    info info
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