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CD112 / Nectin-2 / PVRL2 Antibody, Rabbit MAb

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Human PVRL2/CD112 Antibody Product Information
Immunogen:Recombinant Human CD112 protein (Catalog#10005-H08H)
Clone ID:005
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human CD112 extracellular domain (rh CD112; Catalog#10005-H08H; Met 1-Leu 360; NP_002847.1).
Human PVRL2/CD112 Antibody Usage Guide
Specificity:Human CD112 / Nectin-2 / PVRL2
No cross-reactivity in ELISA with
Mouse PVRL2
Application:ELISA, IHC-P, FCM

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD112 in ELISA.

This antibody can be used at 1-10 μg/mL with the appropriate secondary reagents to detect human CD112 by immunohistochemistry (paraffin-embedded sections).

FCM: 0.5-2 μg/Test

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human PVRL2/CD112 Antibody IHC Application Image
CD112 / Nectin-2 / PVRL2 Antibody, Rabbit MAb, Immunohistochemistry
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Immunohistochemical staining of formalin fixed, paraffin-embedded human lymphonode showing membrane staining of DCs (5 μg/mL).
Human PVRL2/CD112 Antibody FC Application Image
CD112 / Nectin-2 / PVRL2 Antibody, Rabbit MAb, Flow cytometric analysis
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Flow cytometric analysis of anti-Human PVRL2(CD112) on HL60 cells
Other PVRL2/CD112 Antibody Products
CD112/Nectin-2/PVRL2 Background

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.

Human CD112/Nectin-2/PVRL2 References
  • Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.
  • Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
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