|IP||0.2-1 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Mouse PVRL2 was immunoprecipitated using:
Lane A:0.5 mg Raji Whole Cell Lysate0.5 µL anti-Mouse PVRL2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-Mouse PVRL2 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 58 kDa
Observed band size: 58 kDa
Anti-mPVRL2 rabbit monoclonal antibody at 1:500 dilution
Lane A: 293T Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:58 kDa
Observed band size:58 kDa
(We are unsure as to the identity of these extra bands.)
Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Nectin-2 Antibody (PE)||Rabbit||Monoclonal||FCM||10005-R005-P|