Anti-Nectin-2 Antibody

Cat: 50318-R110
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Anti-Nectin-2 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Nectin-2 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse Nectin-2
No cross-reactivity in ELISA with Human CD112 / Nectin-2
Immunogen
Recombinant Mouse PVRL2 protein (Catalog#50318-M08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse PVRL2 extracellular domain (rM PVRL2; Catalog#50318-M08H; NP_033016.3; Met 1-Gly 351).
Source
Monoclonal Rabbit IgG Clone #110
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Nectin-2 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Nectin-2 Antibody (Rabbit Monoclonal antibody) Images
Human Carbonic Anhydrase VIII/CA8 Immunoprecipitation(IP) 7990

Mouse PVRL2 was immunoprecipitated using:

Lane A:0.5 mg Raji Whole Cell Lysate

0.5 µL anti-Mouse PVRL2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse PVRL2 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 58 kDa

Observed band size: 58 kDa

Mouse CD112/Nectin-2/PVRL2 Western blot (WB) 13663

Anti-mPVRL2 rabbit monoclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:58 kDa

Observed band size:58 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Nectin-2 Antibody Alternative Names
Anti-AI325026 Antibody;Anti-AI987993 Antibody;Anti-Cd112 Antibody;Anti-MPH Antibody;Anti-nectin-2 Antibody;Anti-Pvr Antibody;Anti-Pvs Antibody
Nectin-2 Background Information

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.

Full Name
poliovirus receptor-related 2 (herpesvirus entry mediator B)
References
  • Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.
  • Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Nectin-2 Antibody Rabbit Monoclonal ELISA 80327-R013
    Anti-Nectin-2 Antibody Rabbit Polyclonal ELISA 80327-RP01
    Anti-Nectin-2 Antibody Rabbit Polyclonal ELISA,IHC-P 80327-T24
    Anti-Nectin-2 Antibody Rabbit Polyclonal ELISA 50318-RP02
    Anti-Nectin-2 Antibody Rabbit Polyclonal ELISA 50318-RP01
    Anti-Nectin-2 Antibody Mouse Monoclonal ELISA 10005-MM01
    Anti-Nectin-2 Antibody (PE) Rabbit Monoclonal FCM 10005-R005-P
    Anti-Nectin-2 Antibody Rabbit Monoclonal ELISA,ELISA(Cap),FCM 10005-R005
    Anti-Nectin-2 Antibody Rabbit Polyclonal WB,ELISA,IP 10005-RP04
    Anti-Nectin-2 Antibody Rabbit Monoclonal ELISA 10005-R017
    Anti-Nectin-2 Antibody Rabbit Polyclonal ELISA 10005-RP03
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