Anti-Nectin-2 Antibody

Cat: 10005-RP04
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All Nectin-2 Reagents
Anti-Nectin-2 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Nectin-2 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human Nectin-2
Recombinant Human CD112 / Nectin2 protein (Catalog#10005-H08H)
Produced in rabbits immunized with purified, recombinant Human CD112 / Nectin-2 extracellular domain (rhCD112; Catalog#10005-H08H; Met 1-Leu 360; NP_002847.1). CD112 specific IgG was purified by human CD112 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Nectin-2 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Nectin-2 Antibody (Rabbit Polyclonal antibody) Images
Human CD112/Nectin-2/PVRL2 Immunoprecipitation(IP) 4327

CD112 was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

2 µL anti-CD112 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD112 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 58 kDa

Observed band size: 70 kDa

Human CD112/Nectin-2/PVRL2 Western blot (WB) 13034

Anti-CD112 rabbit polyclonal antibody at 1:500 dilution

Lane A: K562 Membrane Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:58 kDa

Observed band size:70 kDa

Anti-Nectin-2 Antibody Alternative Names
Anti-CD112 Antibody;Anti-HVEB Antibody;Anti-Nectin-2 Antibody;Anti-PRR2 Antibody;Anti-PVRR2 Antibody
Nectin-2 Background Information

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.

Full Name
poliovirus receptor-related 2 (herpesvirus entry mediator B)
  • Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.
  • Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
  • Product Description Host Clonality Application Catalog# (PDF)
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    Anti-Nectin-2 Antibody Mouse Monoclonal ELISA 10005-MM01
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