Rat CCR8 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80361-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CCR8 cDNA Clone Datasheet

CCR8 cDNA Clone Product Information

Gene Bank Ref.ID:	XM_236704.3
cDNA Size:	1062
Gene Synonym:	Ccr8
Source:	Rat

CCR8 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus chemokine (C-C motif) receptor 8 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 36 T/C，93 T/C，249 C/T，777 G/A and 927 C/T not causing the amino acid variation.

CCR8 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CCR8 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CCR8 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATTACA CGTTGGAGCC CAATGTCACG ATGACCGACT ACTACCCTGA CTTCTTCACC
61       ACCCCCTGTG ACACAGAGCT CCTCCTCAGG GGCGGCACGT TGTATCTGGC CGTCTTATAC
121      TGCATCTTGT TTGTGCTGGG CCTTCTGGGA AACAGCCTGG TCATCTTGGT CCTTGTGGCC
181      TGCAAGAAAC TGAGGAGTAT CACGGACGTC TACCTCCTGA ACCTGGCCGC TTCTGACCTG
241      CTCTTCGTTC TCTCCATTCC CTTTCAGACC CACAACCTGC TGGACCAGTG GGTGTTTGGG
301      ACCGTGATGT GTAAGGTGGT CTCTGGCCTC TACTACATTG GCTTCTTCAG CAGCATGCTC
361      TTCATCACCC TCATGAGTGT GGACAGGTAC CTGGCTGTCG TCCACCCTGT CCATGCCATC
421      AAAGTGAGGA CGGCCAGAGT GGGCACAGCC CTGAGCCTGG CAGTGTGGCT GGCTGCCATC
481      GCGGCCACCG TCCCACTGAT GGTTTTTTAC CAGGTGTCCT CTGAAGACGG CATGCTACAG
541      TGCTTCCAAC TTTACGACGA GCAGTCTCTG AGGTGGAAGC TCTTCACCCA CTTTGAAGTC
601      AACGCCTTGG GTCTGCTGCT CCCCTTTGCC ATCCTCCTGT TCTGCTACGT CAGGATCCTG
661      CAGCAGCTGC GGGGTTGCCT GAACCACAAC AGGACCAGAG CCATCAAACT GGTGCTCACC
721      ATAGTCGTCG TGTCTTTACT CTTCTGGGTC CCATTCAACG TGGTCCTCTT CCTCACATCC
781      CTGCACGACA TGCACATCTT GGAGGGATGT GCCACCAGGC AGAGGCTGGC CCTGGCCACC
841      CACGTCACGG AGGTCATCTC TTTCATGCAT TGCTGCGTGA ACCCTGTCAT CTATGCTTTC
901      ATCGGAGAGA AGTTCAAGAA GCACCTTGTG GATGTGTTTC AAAAGAGCTG CAGCCACATC
961      TTCCTCTACG TCGGGAGACA GATGCCAGTG GGGGCGTTGG AAAGGCAACT GTCCTCGAAC
1021     CAGCGATCTT CCCACTCTTC CACACTGGAC TACATCTTGT AA

Quality Control:cDNA sequencing results is available upon request.

Cytokine