Mouse CCR8 Gene cDNA Clone / ORF Clone

CCR8 cDNA Clone Datasheet

CCR8 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_007720.2
• cDNA Size: 1062
• Gene Synonym: mCCR8; Cmkbr8; MGC123958; MGC123959; Ccr8
• Source: Mouse

CCR8 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus chemokine (C-C motif) receptor 8 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 261 T/C not causing the amino acid variation.

CCR8 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CCR8 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CCR8 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATTACA CGATGGAGCC CAACGTCACG ATGACCGACT ACTACCCTGA TTTCTTCACC
61       GCCCCCTGTG ACGCAGAGTT CCTCCTCAGG GGCAGCATGC TGTATCTGGC CATCTTGTAC
121      TGCGTCTTGT TTGTGCTGGG CCTTCTGGGG AACAGCCTGG TCATCTTAGT CCTCGTGGGC
181      TGCAAGAAAC TGAGGAGCAT CACAGATATC TACCTCCTGA ACCTGGCCGC ATCCGACCTG
241      CTCTTTGTCC TCTCTATTCC CTTTCAGACC CACAACCTGC TGGACCAGTG GGTGTTTGGG
301      ACTGCGATGT GTAAGGTGGT CTCTGGCCTT TATTACATTG GTTTTTTCAG CAGTATGTTC
361      TTCATCACCC TAATGAGTGT GGACAGGTAT CTGGCTATTG TCCACGCTGT CTATGCCATC
421      AAGGTGAGGA CGGCCAGCGT GGGCACAGCC CTGAGTCTGA CAGTGTGGCT GGCTGCTGTC
481      ACAGCCACCA TCCCCTTGAT GGTTTTTTAC CAAGTGGCCT CTGAAGACGG CATGCTACAA
541      TGTTTCCAGT TTTATGAAGA GCAGTCTTTG AGGTGGAAGC TCTTTACCCA CTTTGAAATC
601      AACGCCTTGG GTCTGCTGCT CCCCTTTGCC ATCCTCCTGT TCTGCTATGT CAGGATCCTG
661      CAGCAGCTGC GGGGCTGCCT GAACCACAAC AGGACCAGAG CCATCAAGCT GGTGCTCACC
721      GTAGTCATTG TGTCTTTACT CTTCTGGGTC CCATTCAACG TGGCCCTTTT CCTCACGTCC
781      CTGCACGACC TGCACATCTT GGATGGATGT GCCACGAGGC AGAGGCTGGC TCTGGCCATC
841      CATGTCACAG AGGTCATCTC TTTTACCCAC TGCTGCGTGA ACCCCGTCAT CTACGCGTTC
901      ATAGGAGAGA AGTTTAAGAA ACACCTCATG GATGTGTTTC AAAAGAGCTG CAGCCACATC
961      TTCCTCTACT TAGGGAGACA AATGCCCGTG GGGGCGTTGG AAAGGCAGCT GTCCTCGAAC
1021     CAGCGATCTT CCCATTCTTC CACCCTGGAT GACATCTTGT AA

 

Quality Control:cDNA sequencing results is available upon request.