Rat CCR4 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80357-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CCR4 cDNA Clone Datasheet

CCR4 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_133532.2
cDNA Size:	1083
Gene Synonym:	Cmkbr4; Ccr4
Source:	Rat

CCR4 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus chemokine (C-C motif) receptor 4 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 977 C/T resulting in the amino acid Pro substitution by Leu.

CCR4 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CCR4 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CCR4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAATGCCA CAGATGTCAC AGACACCACC CAGGATGAAG CCGCGTACAA TAGTTACTAC
61       TTCGACGAGA GCTTGCCAAA GCCTTGCACC AAGGAAGGCA TCAAGGCGTT CGGCGAGGTC
121      TTCCTGCCTC CTCTCTACTC CTTGGTCTTT CTGTTGGGTC TGTTTGGGAA TTCTGTTGTG
181      GTTCTGGTGC TGTTCAAGTA CAAGAGGCTC AAGTCCATGA CTGACGTGTA CCTGCTGAAC
241      CTAGCCATCT CGGATTTACT GTTTGTGCTG TCTCTCCCGT TCTGGGGCTA CTATGCTGCT
301      GACCAGTGGG TTTTTGGTCT AGGTCTGTGT AAGATTATCT CATGGATGTA CCTGGTGGGC
361      TTCTACAGCG GGATCTTCTT CATCATGCTC ATGAGTATAG ACAGATACCT GGCCATCGTG
421      CACGCAGTGT TTTCCTTGAG AGCGAGGACT CTGACCTATG GGGTCATCAC CAGCCTCATC
481      ACGTGGTCTG TGGCTGTGTT TGCCTCCCTA CCAGGCCTCT TGTTCAGCAC CTGCGACACA
541      GAGAACAACC ACACATACTG CAAAACCCAG TACTCGGTCA ACTCGACCAC GTGGAAAGTC
601      CTCAGCTCCC TGGAGATCAA TGTCTTGGGG CTGGTCATCC CCCTGGGGAT CATGTTGTTC
661      TGCTATTCCA TGATCATCAG GACCCTGAGA CACTGCAAGA ATGAGAAGAA GAACAGAGCA
721      GTTCGGATGA TCTTCGCCGT GGTGGTCCTC TTCCTCGGCT TCTGGACGCC GTACAACGTG
781      GTGCTTTTCC TGGAGACCTT GGTGGAGCTC GAAGTCCTTC AGGACTGCAC CTTGGAGAGG
841      TACCTAGACT ATGCCATCCA GGCCACAGAA ACCCTGGCCT TCATTCACTG CTGCCTTAAC
901      CCTGTCATTT ACTTCTTCCT TGGGGAGAAA TTCCGCAAGT ACATCGCCCA ACTCTTCAGA
961      ACGTGCCGGG GTCCTCTTGT GCTCTGCCAA CACTGTGACT TCCTTCAGAT CTCCTCGGCC
1021     GACACTTCCA GCTCCTCTTA CACTCAGTCC ACTGTGGATC ATGACTTCCG TGACGCTTTG
1081     TAA

Quality Control:cDNA sequencing results is available upon request.

Cytokine