Rat CCR3 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80356-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CCR3 cDNA Clone Datasheet

CCR3 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_053958.1
cDNA Size:	1080
Gene Synonym:	Cmkbr3; Ccr3
Source:	Rat

CCR3 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus chemokine (C-C motif) receptor 3 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Same as Gene Bank Ref. ID sequence with the nucleotide 955 T deletion mutation.

CCR3 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CCR3 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CCR3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCATCCA ACGAAGAGGA ACTCAAGACT GTTGTTGAAA CCTTTGAGAC CACACCCTAT
61       GAATATGAGT GGGCACCACC CTGTGAAAAA GTCAGCATCA GAGAGCTGGG GTCATGGCTC
121      CTGCCTCCAC TGTATTCCCT GGTGTTCATC GTCGGCCTCC TGGGCAACAT GATGGTTGTG
181      TTGATCCTCA TAAAGTACAG GAAGCTGCAA ATTATGACTA ATATCTACCT ACTCAACTTG
241      GCAATTTCTG ACCTGCTCTT TCTCTTCACT GTCCCATTCT GGATTCACTA TGTTCTGTGG
301      AATGAGTGGG GTTTTGGCCA CTGCATGTGT AAAATGCTCT CTGGGCTTTA TTACCTGGCC
361      TTGTACAGCG AGATCTTTTT CATCATCCTG CTGACAATCG ACAGGTACCT GGCTATCGTT
421      CATGCTGTGT TGGCCCTTCG AGCACGAACT GTGACTTTTG CTACTATCAC CAGCATCATC
481      ACTTGGGGTC TTGCAGTATT GGCAGCATTG CCTGAATTTA TCTTCCATGA GTCCCAAGAC
541      AATTTTGGAG ATCTTTCCTG CAGTCCTCGC TATCCAGAGG GCGAAGAAGA CAGCTGGAAG
601      CGTTTCCATG CTCTAAGAAT GAACATTTTT GGTTTAGCTC TTCCTCTCCT CATTATGGTT
661      ATCTGCTACT CAGGAATCAT TAAAACTCTG CTTAGATGCC CCAATAAAAA AAAACACAAG
721      GCCATCCAGC TTATTTTTGT TGTCATGATA GTCTTTTTTA TTTTTTGGAC TCCATACAAC
781      CTGGTTCTTC TTCTGTCTGC TTTCCACAGC ACATTTTTGG AGACCAGCTG TCAGCAGAGC
841      ATACACCTGG ACCTGGCCAT GCAAGTGACT GAGGTGATTA CCCACACCCA CTGCTGTATC
901      AATCCAATAA TCTATGCCTT TGTTGGTGAG AGGTTCCGGA AACACCTTCG GCTCTTTTTC
961      CACAGAAATG TGGCAATCTA CCTGAGAAAA TATATTTCAT TCCTTCCTGG CGAGAAACTG
1021     GAAAGAACAA GCTCTGTCTC CCCATCAACT GGGGAGCAAG AAATCTCTGT GGTGTTTTAG

Quality Control:cDNA sequencing results is available upon request.

Cytokine