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Human CCN3 / NOV / IGFBP9 ELISA Pair Set

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Materials provided
Capture Ab:0.5 mg/mL of rabbit anti-NOV monoclonal antibody, Dilute to a working concentration of 2.0 μg/mL in CBS before coating.
Detection Ab:0.5 mg/mL mouse anti-NOV monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.05 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 100 ng of recombinant NOV. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
Sensitivity
The minimum detectable dose of Human CCN3 / NOV / IGFBP9 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Principle of the product
The Human CCN3 / NOV / IGFBP9 ELISA Pair Set is for the quantitative determination of Human CCN3 / NOV / IGFBP9.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CCN3 / NOV / IGFBP9 coated on a 96-well plate. Standards and samples are added to the wells, and any CCN3 / NOV / IGFBP9 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-CCN3 / NOV / IGFBP9 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CCN3 / NOV / IGFBP9 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Storage
Capture Antibody: Aliquot and store at -20℃ to -70℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -70℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20℃ to -70℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -20℃ to -70℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Image
Human CCN3 / NOV / IGFBP9 ELISA standard curve

Background
References
  • Manara MC, et al. (2002) The expression of ccn3(nov) gene in musculoskeletal tumors. Am J Pathol. 160(3): 849-59.
  • Lin CG, et al. (2003) CCN3 (NOV) is a novel angiogenic regulator of the CCN protein family. J Biol Chem. 278(26): 24200-8.
  • Vallacchi V, et al. (2009) CCN3/nephroblastoma overexpressed matricellular protein regulates integrin expression, adhesion, and dissemination in melanoma. Cancer Res. 68(3): 715-23.
  • Sin WC, et al. (2009) Matricellular protein CCN3 (NOV) regulates actin cytoskeleton reorganization. J Biol Chem. 284(43): 29935-44.
  • McCallum L, et al. (2009) CCN3--a key regulator of the hematopoietic compartment. Blood Rev. 23(2): 79-85.
  • Insulin-like Growth Factor (IGF) Related Products
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    • Human CCN3 / NOV / IGFBP9 ELISA standard curve
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