NOV / CCN3 Matched ELISA Antibody Pair Set,Human General Information
NOV / CCN3 Matched ELISA Antibody Pair Set,Human
Solid Phase Sandwich ELISA
Quantitative determination of Human NOV / CCN3
1. Capture Antibody 0.5 mg/mL of rabbit anti-Human CCN3 / NOV / IGFBP9 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10264-R130)
2. Detection Antibody 0.2 mg/mL of mouse anti-Human CCN3 / NOV / IGFBP9 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.3 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10264-MM09)
3. Standard Each vial contains 47 ng of recombinant Human CCN3 / NOV / IGFBP9. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.
This NOV / CCN3 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human NOV / CCN3 . It contains Human NOV / CCN3 capture antibody, Human NOV / CCN3 detector antibody
and a highly purified
recombinant Human NOV / CCN3 protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
NOV / CCN3 Matched ELISA Antibody Pair Set,Human Images
NOV / CCN3 Matched ELISA Antibody Pair Set,Human Alternative Names
CCN3 Matched ELISA Antibody Pair Set, Human;IBP-9 Matched ELISA Antibody Pair Set, Human;IGFBP-9 Matched ELISA Antibody Pair Set, Human;IGFBP9 Matched ELISA Antibody Pair Set, Human;NOVh Matched ELISA Antibody Pair Set, Human
NOV / CCN3 Background Information
Protein NOV homolog, also known as Nephroblastoma-overexpressed gene protein homolog, NOV, and CCN3, is a putative ligand for integrin receptors, is tightly associated with the extracellular matrix and mediates diverse cellular functions, including cell adhesion and proliferation. CCN3 has been shown to negatively regulate growth although it promotes migration in a cell type-specific manner. This secreted protein belongs to the CCN family, and its expression was observed in a broad variety of tissues from the early stage of development , and altered expression of CCN3 has been observed in a variety of tumors, including hepatocellular carcinomas, Wilm's tumors, Ewing's sarcomas, gliomas, rhabdomyosarcomas, and adrenocortical carcinomas. Mature CCN3 protein has five distinct modules and truncated protein variants with altered function are found in many cancers. CCN3 acts through the core stem cell signalling pathways including Notch and Bone Morphogenic Protein, connecting CCN3 with the modulation of self-renewal and maturation of a number of cell lineages including hematopoietic, osteogenic and chondrogenic. CCN3 may affect the extracellular environment of the niche for hematopoietic stem cells. CCN3 has emerged as a key player in stem cell regulation, hematopoiesis and a crucial component within the bone marrow microenvironment.
Manara MC, et al. (2002) The expression of ccn3(nov) gene in musculoskeletal tumors. Am J Pathol. 160(3): 849-59.Lin CG, et al. (2003) CCN3 (NOV) is a novel angiogenic regulator of the CCN protein family. J Biol Chem. 278(26): 24200-8.Vallacchi V, et al. (2009) CCN3/nephroblastoma overexpressed matricellular protein regulates integrin expression, adhesion, and dissemination in melanoma. Cancer Res. 68(3): 715-23.Sin WC, et al. (2009) Matricellular protein CCN3 (NOV) regulates actin cytoskeleton reorganization. J Biol Chem. 284(43): 29935-44.McCallum L, et al. (2009) CCN3--a key regulator of the hematopoietic compartment. Blood Rev. 23(2): 79-85.