Anti-NOV / CCN3 Antibody (Rabbit Polyclonal antibody) General Information
Anti-NOV / CCN3 Antibody
Reacts with: Human
Human NOV / CCN3
Recombinant Human CCN3 / NOV / IGFBP9 protein (Catalog#10264-H08B)
Produced in rabbits immunized with purified, recombinant Human CCN3 / NOV / IGFBP9 (rh CCN3 / NOV / IGFBP9; Catalog#10264-H08B; NP_002505.1; Met 1-Met 357). CCN3 / NOV / IGFBP9 specific IgG was purified by Human CCN3 / NOV / IGFBP9 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-NOV / CCN3 Antibody (Rabbit Polyclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-NOV / CCN3 Antibody (Rabbit Polyclonal antibody) Images
Immunochemical staining of human NOV in human brain with rabbit polyclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Immunochemical staining of human NOV in human brain (from 2 donors) with rabbit polyclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections.
Anti-NOV rabbit polyclonal antibody at 1:500 dilution
Lane A: K562 Whole Cell Lysate
Lysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:39 kDa
Observed band size:37 kDa
Anti-NOV / CCN3 Antibody Alternative Names
Anti-CCN3 Antibody;Anti-IBP-9 Antibody;Anti-IGFBP-9 Antibody;Anti-IGFBP9 Antibody;Anti-NOVh Antibody
NOV / CCN3 Background Information
Protein NOV homolog, also known as Nephroblastoma-overexpressed gene protein homolog, NOV, and CCN3, is a putative ligand for integrin receptors, is tightly associated with the extracellular matrix and mediates diverse cellular functions, including cell adhesion and proliferation. CCN3 has been shown to negatively regulate growth although it promotes migration in a cell type-specific manner. This secreted protein belongs to the CCN family, and its expression was observed in a broad variety of tissues from the early stage of development , and altered expression of CCN3 has been observed in a variety of tumors, including hepatocellular carcinomas, Wilm's tumors, Ewing's sarcomas, gliomas, rhabdomyosarcomas, and adrenocortical carcinomas. Mature CCN3 protein has five distinct modules and truncated protein variants with altered function are found in many cancers. CCN3 acts through the core stem cell signalling pathways including Notch and Bone Morphogenic Protein, connecting CCN3 with the modulation of self-renewal and maturation of a number of cell lineages including hematopoietic, osteogenic and chondrogenic. CCN3 may affect the extracellular environment of the niche for hematopoietic stem cells. CCN3 has emerged as a key player in stem cell regulation, hematopoiesis and a crucial component within the bone marrow microenvironment.
Manara MC, et al. (2002) The expression of ccn3(nov) gene in musculoskeletal tumors. Am J Pathol. 160(3): 849-59.Lin CG, et al. (2003) CCN3 (NOV) is a novel angiogenic regulator of the CCN protein family. J Biol Chem. 278(26): 24200-8.Vallacchi V, et al. (2009) CCN3/nephroblastoma overexpressed matricellular protein regulates integrin expression, adhesion, and dissemination in melanoma. Cancer Res. 68(3): 715-23.Sin WC, et al. (2009) Matricellular protein CCN3 (NOV) regulates actin cytoskeleton reorganization. J Biol Chem. 284(43): 29935-44.McCallum L, et al. (2009) CCN3--a key regulator of the hematopoietic compartment. Blood Rev. 23(2): 79-85.