CCL7 / MCP3 Protein (His Tag)

Chemokine (C-C motif) ligand 7 Protein Datasheet

CCL7 / MCP3 Protein Product Information

• Synonym: FIC, MARC, MCP-3, MCP3, MGC138463, MGC138465, NC28, SCYA6, SCYA7
• Protein Construction: A DNA sequence encoding the mature form of human CCL7 (NP_006264.2) (Gln 24-Leu 99) was expressed, with an initial Met at the N-terminus and a polyhistide tag at the C-terminus.
• Source: Human
• Expression Host: E. Coli

CCL7 / MCP3 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human CCL7 consisting of 87 amino acids and has a calculated molecular mass of 10.5 kDa. It migrates as an approximately 13 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 50mM Tris, 30%glycerol, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CCL7 / MCP3 protein

CCL7 / MCP3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CCL7 / MCP3 Protein Description

C-C motif chemokine 7, also known as Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, MCP-3, Small-inducible cytokine A7, CCL7 and MCP3, is a secreted protein which belongs to the?intercrine beta (chemokine CC) family. Chemokines and chemokine receptors are pivotal regulators of cellular communication and trafficking. In addition to the approximately 20 chemokine receptors that have been cloned and described, various orphan receptors with a chemokine receptor-like structure are known. CCL7 / MCP3 is a chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. It augments monocyte anti-tumor activity. CCL7 / MCP3 also induces the release of gelatinase B. This protein can bind heparin. It also binds to CCR1, CCR2 and CCR3. Monocyte chemotactic protein 3 (MCP-3/CCL7), a CC chemokine able to attract and activate a large panel of leukocytes including natural killer cells and T lymphocytes, could be beneficial in antitumor therapy. CCL7 and CXCL10 play a critical role in mediating oxidative stress-induced neutrophilic airway inflammation.

References

1. Michalec,L. et al., 2002, J Immunol. 168 (2):846-52.
2. Biber,K. et al., 2003, J Leukoc Biol. 74 (2):243-51.
3. Wetzel,K. et al., 2007, Int J Cancer. 120 (6):1364-71.
4. Hintzen,C. et al., 2008, J Immunol.181 (10):7341-9.