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Cynomolgus monkey CCL25 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog Vector (Price) Quantity In Stock Operation
CG90139-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CCL25 cDNA Clone Datasheet

 

CCL25 cDNA Clone Product Information

cDNA Size:

450

Gene Synonym:

CCL25

Source: Cynomolgus monkey

CCL25 cDNA Clone Description

cDNA Description:

ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) chemokine (C-C motif) ligand 25 DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 240 C/A resulting in the amino acid Asn substitution by Lys and 132 G/A, 294 C/A not causing the amino acid variation.
Please check the sequence information before order.

CCL25 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CCL25 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to incubation at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CCL25 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAACCTGT GGCTCCTGGC TTGCCTGGTG GCCGGCTTCC TGGGAGCCTG GGCCCCCACT
61       GTCCACACCC AAGGTGTCTT TGAGGACTGC TGCCTGGCCT ACCACTACCC CATTGGGTGG
121      GCTGTGCTCC GACACGCCTG GACTTATCGG ATCCAGGAGG TGAGCGGGAG CTGCAATCTG
181      CCTGCTGCAA TATTCTACCT CCCCAAGAGA CACAGGAAAG TGTGTGGGAA CCCCAAGAAA
241      AAGGAGGTGC AGAGAGCCAT GAAGCTCCTG GATGCTCGCA ATAAGATTTT TGCAAAGTTC
301      CGCCACAACA CGCAGACCTT CCCAGGCTCT CATGCTGCAA AGAAGTTGAG TTCTGCAAAC
361      TCCAAACTAC CATCGTCTAA GTTTAGCAAT CCCACCAGCA GCAGCAAGAG GAATGCCTCC
421      CTCCTGACAT CAGCTAATTC AGGACTGTGA

 

Quality Control:cDNA sequencing results is available upon request.