CCL24 / Eotaxin-2 / MPIF-2 Protein (His Tag) (Cytokine)

Chemokine (C-C motif) ligand 24 Protein Datasheet

CCL24 / Eotaxin-2 / MPIF-2 Protein Product Information

• Synonym: Ckb-6, MPIF-2, MPIF2, SCYA24
• Protein Construction: A DNA sequence encoding the human CCL24 (O00175) （Met 1-Cys 119）was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

CCL24 / Eotaxin-2 / MPIF-2 Protein QC Testing

• Purity: > 87 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Val 27
• Molecular Mass: The secreted recombinant human CCL24 consists of 103 amino acids and predicts a molecular mass of 11.8 kDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10%gly.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CCL24 / Eotaxin-2 / MPIF-2 protein

CCL24 / Eotaxin-2 / MPIF-2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CCL24 / Eotaxin-2 / MPIF-2 Protein Description

CCL24, also known as Eotaxin-2 and MPIF-2, is a member of the CC chemokine family. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. It is produced by activated monocytes and T lymphocytes. CCL24 is selectively chemoattracts for the following cells: eosinophils, basophils, Th2 T cells, mast cells, and certain subsets of dendritic cells. It is strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils. CCL24 is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line and binds to CCR3. Eotaxin-2 also inhibits the proliferation of multipotential hematopoietic progenitor cells. Eotaxin-2 has been shown that elevated levels of eotaxin-2 has been seen in patients with aspirin-exacerbated respiratory disease (AERD), such as asthma. People with lower plasma levels of eotaxin-2 have not been showing tendency to develop aspirin inducible asthma.

References

1. Manousou P. et al., 2010, Clin Exp Immunol. 162 (2): 337-47.
2. Owczarek W. et al., 2010, Cytokine. 50 (2): 181-5.
3. Luesink M. et al., 2009, Blood. 114 (27): 5512-21.