Mouse CCL20 Gene cDNA Clone / ORF Clone (Cytokine)

CCL20 cDNA Clone Datasheet

CCL20 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_016960.1
• cDNA Size: 294
• Gene Synonym: CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1; Ccl20
• Source: Mouse

CCL20 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus chemokine (C-C motif) ligand 20 DNA.
• Vector: Cloning Vector: pMD18-T Simple Vector.

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID.

CCL20 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CCL20 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

CCL20 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCTGCG GTGGCAAGCG TCTGCTCTTC CTTGCTTTGG CATGGGTACT GCTGGCTCAC
61       CTCTGCAGCC AGGCAGAAGC AGCAAGCAAC TACGACTGTT GCCTCTCGTA CATACAGACG
121      CCTCTTCCTT CCAGAGCTAT TGTGGGTTTC ACAAGACAGA TGGCCGATGA AGCTTGTGAC
181      ATTAATGCTA TCATCTTTCA CACGAAGAAA AGAAAATCTG TGTGCGCTGA TCCAAAGCAG
241      AACTGGGTGA AAAGGGCTGT GAACCTCCTC AGCCTAAGAG TCAAGAAGAT GTAA

 

Quality Control:cDNA sequencing results is available upon request.