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Mouse CCL20 Gene cDNA Clone (full-length ORF Clone)

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CCL20cDNA Clone Product Information
Gene Bank Ref.ID:NM_016960.1
cDNA Size:294
cDNA Description:ORF Clone of Mus musculus chemokine (C-C motif) ligand 20 DNA.
Gene Synonym:CKb4, LARC, ST38, MIP3A, MIP-3A, Scya20, MIP-3[a], exodus-1, Ccl20
Species:Mouse
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Shipping Carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Chemokine & Receptor Related Products
Product nameProduct name
Human CXCL2 / MIP-2 ProteinRat CXCL1 / MGSA / NAP-3 ProteinRat CXCL2 / MIP-2 ProteinHuman CCL2 / MCP-1 / MCP1 Protein (His Tag)Human CCL23 / MIP 3 Protein (His Tag)Mouse NAP-2 / PPBP / CXCL7 Protein (aa 49-109, His Tag)Mouse NAP-2 / PPBP / CXCL7 Protein (aa 40-113, His Tag)Mouse CCL22 / MDC Protein (His Tag)Mouse CXCL14 / BRAK ProteinMouse CXCL1 / MGSA / NAP-3 ProteinHuman NAP-2 / PPBP / CXCL7 Protein (His Tag)Human IL-8 / CXCL8 Protein (aa 28-99)Human CCL13 / MCP-4 Protein (His Tag)Human CCL18 / PARC / MIP4 Protein (His Tag)Mouse CCL2 / MCP-1 / MCP1 Protein (His Tag)Human CCL16 / HCC-4 / NCC4 Protein (His Tag)Human CCL16 / HCC-4 / NCC4 Protein (His Tag)Human CCL4 / MIP1B Protein (His Tag)Human CCL3 / Mip1a Protein (His Tag)Human I-309 / CCL1 / TCA-3 Protein (Fc Tag)Human Fractalkine / CX3CL1 Protein (His Tag)Human CXCL12 / SDF1b Protein (Fc Tag)Human CXCL4 / PF4 ProteinHuman IL-8 / CXCL8 Protein (aa 23-99, Fc Tag)Human IL-8 / CXCL8 Protein (aa 23-99)Human IL-8 / CXCL8 Protein (aa 28-99, Fc Tag)Human IL-8 / CXCL8 Protein (aa 28-99)Human CXCL3 / GRO gamma Protein (His Tag)Human CCL5 / RANTES Protein (His & mucin Tag)Mouse CXCL16 / SR-PSOX Protein (His Tag)Human MCP-3 / CCL7 Protein (His Tag)Human CCL8 / MCP-2 Protein (SUMO Tag)Human CXCL1 / MGSA / NAP-3 Protein (His & SUMO Tag)Human CCL17 / TARC / SCYA17 Protein (His Tag)Mouse CXCL2 / GRO2 / MIP-2 (His & SUMO Tag)Human CCL15 / MIP-5 / MIP-1 delta Protein (aa 22-113, His Tag)Human CCL17 / TARC / SCYA17 ProteinMouse CCL8 / MCP-2 Protein (His & NusA Tag)Mouse CCL6 / C-C motif ligand 6 Protein (His Tag)Cynomolgus CXCL13 / BCA-1 / BLC Protein (His Tag)Human CXCL1 / MGSA / NAP-3 Protein (His & NusA Tag)Cynomolgus CXCL12 / SDF-1 Protein (Fc Tag)Mouse CXCL9 / MIG / C-X-C motif chemokine 9 ProteinHuman CXCL9 / MIG / C-X-C motif chemokine 9 ProteinHuman CCL21 / 6Ckine ProteinHuman CCL14 / HCC-1 / HCC-3 Protein (His Tag)Mouse CCL17 / TARC ProteinCynomolgus / Rhesus Fractalkine / CX3CL1 Protein (Fc Tag)Human CCL14 / HCC-1 / HCC-3 Protein (aa 28-93, His Tag)Human MCP-3 / CCL7 Protein (His Tag)Mouse I-309 / CCL1 / TCA-3 Protein (Fc Tag)Human CCL11 Protein (His Tag)Human CXCL12 / SDF-1 ProteinMouse XCL1 Protein (His Tag)Rat NAP-2 / PPBP / CXCL7 Protein (Fc Tag)Human XCL1 Protein (His Tag)Human CCL15 / MIP-5 / MIP-1 delta Protein (aa 46-113, His Tag)Human XCL2 Protein (His Tag)Human CXCL12 / SDF-1 Protein (isoform a, His Tag)Rat CXCL16 / SR-PSOX Protein (His Tag)Cynomolgus XCL1 Protein (His Tag)Human CCL22 / MDC Protein (His Tag)Mouse CCL20 / MIP-3 alpha Protein (His Tag)Human CCL24 / Eotaxin-2 / MPIF-2 Protein (His Tag)Human CXCL12 / SDF-1 Protein (isoform a)Cynomolgus CCL21 / 6Ckine ProteinHuman CXCL1 / MGSA / NAP-3 ProteinHuman FAM19A2 Protein (Fc Tag)Human CXCL10 / Crg-2 ProteinCanine IL-8 / CXCL8 ProteinRat CXCL16 / SR-PSOX Protein (Fc Tag)Cynomolgus NAP-2 / PPBP / CXCL7 Protein (Fc Tag)Human CXCL5 ProteinHuman I-309 / CCL1 / TCA-3 ProteinMouse CCL3 / Mip1a ProteinCynomolgus CCL17 / TARC Protein (His Tag)Mouse CXCL12 / SDF-1 ProteinCynomolgus CXCL9 / MIG / C-X-C motif chemokine 9 ProteinCynomolgus IL-8 / CXCL8 ProteinCanine CXCL12 / SDF-1 ProteinCanine CXCL13 / BCA-1 Protein (His Tag)Human CCL28 Protein (His Tag)Canine CXCL16 / SR-PSOX Protein (His Tag)Human CCL27 / CTACK Protein (His Tag)Human CXCL14 / BRAK ProteinHuman CCL23 / MIP 3 Protein (His Tag)Human CCL23 / MIP 3 ProteinCanine CXCL16 / SR-PSOX Protein (Fc Tag)Canine Fractalkine / CX3CL1 Protein (Fc Tag)Canine Fractalkine / CX3CL1 ProteinCanine XCL1 Protein (His Tag)Human CCL20 / MIP-3 alpha Protein (His Tag)Rat CCL3 / Mip1a ProteinHuman FAM19A4 / TAFA4 Protein (Fc Tag)Mouse MCP-3 / CCL7 Protein (His Tag)Human CCL5 / RANTES Protein
Background

Chemokine (C-C motif) ligand 20 (CCL20) or liver activation regulated chemokine (LARC) or Macrophage Inflammatory Protein-3 (MIP3A) is a small cytokine belonging to the CC chemokine family that attracts immature dendritic cells and memory T lymphocytes, both expressing CCR6. Depending on the cell type, this chemokine was found to be inducible by cytokines (IL-1beta) and by bacterial, viral, or plant products (including LPS, dsRNA, and PMA). MIP3A / CCL20 is Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of MIP3A / CCL20 has been seen in thymus, prostate, testis, small intestine and colon. As a chemotactic factor, MIP3A / CCL20 attracts lymphocytes and, slightly, neutrophils, but not monocytes. This chemokine may Inhibit proliferation of myeloid progenitors in colony formation assays and it may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. Its C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Chemokine CCL20 was shown to play a role in colorectal cancer (CRC) pathogenesis.

References
  • Vicinus B, et al. (2012) miR-21 functionally interacts with the 3'UTR of chemokine CCL20 and down-regulates CCL20 expression in miR-21 transfected colorectal cancer cells. Cancer Lett. 316(1): 105-12.
  • Ding X, et al. (2012) High Expression of CCL20 Is Associated with Poor Prognosis in Patients with Hepatocellular Carcinoma after Curative Resection. J Gastrointest Surg. 16(4): 828-36.
  • Ivison SM, et al. (2010) Oxidative stress enhances IL-8 and inhibits CCL20 production from intestinal epithelial cells in response to bacterial flagellin. Am J Physiol Gastrointest Liver Physiol. 299(3): 733-41.
  • Catalog:MG50048-M
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