Human CCL2/MCP-1 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10134-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID: NM_002982.3

cDNA Size: 299

Synonym: HC11, MCAF, MCP1, MCP-1, SCYA2, GDCF-2, SMC-CF, HSMCR30, MGC9434

Source: Human.

Description

cDNA Description: ORF Clone of Homo sapiens chemokine (C-C motif) ligand 2 DNA

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide 10T deletion mutation.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGAAAGTTC TGCCGCCCTT CTGTGCCTGC TGCTCATAGC AGCCACCTTC ATTCCCCAAG
61       GGCTCGCTCA GCCAGATGCA ATCAATGCCC CAGTCACCTG CTGTTATAAC TTCACCAATA
121      GGAAGATCTC AGTGCAGAGG CTCGCGAGCT ATAGAAGAAT CACCAGCAGC AAGTGTCCCA
181      AAGAAGCTGT GATCTTCAAG ACCATTGTGG CCAAGGAGAT CTGTGCTGAC CCCAAGCAGA
241      AGTGGGTTCA GGATTCCATG GACCACCTGG ACAAGCAAAC CCAAACTCCG AAGACTTGA

Quality Control: cDNA sequencing results is available upon request.

Cytokine

Chemokine