Cynomolgus monkey CCL17 Gene cDNA Clone / ORF Clone (Cytokine)

CCL17 cDNA Clone Datasheet

CCL17 cDNA Clone Product Information

• cDNA Size: 285
• Gene Synonym: CCL17
• Source: Cynomolgus monkey

CCL17 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) chemokine (C-C motif) ligand 17 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 84 C/T and 198 T/C not causing the amino acid variation.
  Please check the sequence information before order.

CCL17 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CCL17 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CCL17 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCCCAC TGAAGATGCT GGCCCTGGTC ATCCTCCTCC TGGGGGCTTC TCTGCAGCAC
61       ATCCATGCAG CTCGAGGGAC CAATGTGGGC CGGGAGTGCT GCCTGAAGTA CTTCAAGGGA
121      GCCATTCCCC TTAGAAAGCT GAAGACGTGG TACCAGACAT CCGAGGACTG TTCCAGGGAT
181      GCCATTGTTT TTGTAACCGT CCAGAACAAG GCCATCTGTT CGGACCCCAA CGACAAGAAA
241      GTGAAGAAGG CACTTAAATA CCTGCAAAGC CTTGAGAGGT CTTGA

 

Quality Control:cDNA sequencing results is available upon request.