CCL1 / I-309 / TCA3 Protein (Native) (Cytokine)

Chemokine (C-C motif) ligand 1 Protein Datasheet

CCL1 / I-309 / TCA3 Protein Product Information

• Synonym: CCL1, SCYA1
• Protein Construction: A DNA sequence encoding the human CCL1 (P22362) (Lys24-Lys96) was expressed, with a N-terminal Met.
• Source: Human
• Expression Host: E.coli

CCL1 / I-309 / TCA3 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human CCL1 consists of 75 amino acids and predicts a molecular mass of 8.7 KDa. It migrates as an approximately 9 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 40% acetonitrile, 0.1%TFA 20% H2O.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CCL1 / I-309 / TCA3 protein

CCL1 / I-309 / TCA3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CCL1 / I-309 / TCA3 Protein Description

Chemokines are a family of structurally related leukocyte chemoattractant cytokines that play a central role during immunoregulatory and inflammation processes. All chemokines contain four conserved cysteines linked by disulfide bonds, and two major subfamilies, namely CXC and CC, are defined on the basis of the first two cysteines which are separated by one amino acid or are adjacent. Human CCL1, also well known as I-309, is a small glycoprotein (73 amino acid residue as mature form) secreted by activated T lymphocytes. Human I-309 is assumed to be homologous to the mouse TCA-3 despite of the only approximately 42% amino acid sequence identity. CCL1 interacts with the G protein-linked transmembrane chemokine receptors CCR8, and induces biochemical events that may result in the control of chemotaxis, proliferation, apoptosis and adhesion. It has been demonstrated that CCL1 displays chemotactic activity for monocytes and other cell types such as NK cells and dendritic cells, but not for neutrophils. Furthermore, as the only known physiological ligand for CCR8, CCL1 was identified as a potent inhibitor of HIV-1 envelope-mediated cell-cell fusion and virus infection.

References

1. Miller MD. et al., 1992, Proc Natl Acad Sci. 89: 2950-4.
2. Roos RS. et al., 1997, J Biol Chem. 272: 17251-4.
3. Bernardini G. et al., 2000, Blood. 96: 4039-45.
4. Horuk R. et al., 1998, J Biol Chem. 273: 386-91.
5. Spinetti G. et al., 2003, J Leuko Biol. 73: 201-7.