Mouse CCL1 / I-309 / TCA3 Protein (Cytokine)

Chemokine (C-C motif) ligand 1 Protein Datasheet

CCL1 / I-309 / TCA3 Protein Product Information

• Synonym: RP23-446K18.2, BF534335, I-309, Scya1, Tca-3
• Protein Construction: A DNA sequence encoding the secreted form of mouse CCL1 isoform 1 (P10146-1) (Lys 24-Cys 92) was fused with the Fc region of human IgG1 at the N-terminus.
• Source: Mouse
• Expression Host: Human Cells

CCL1 / I-309 / TCA3 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu
• Molecular Mass: The recombinant mouse CCL1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 330 amino acids and has a calculated molecular mass of 36.2 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 44 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CCL1 / I-309 / TCA3 protein

CCL1 / I-309 / TCA3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CCL1 / I-309 / TCA3 Protein Description

Chemokines are a family of structurally related leukocyte chemoattractant cytokines that play a central role during immunoregulatory and inflammation processes. All chemokines contain four conserved cysteines linked by disulfide bonds, and two major subfamilies, namely CXC and CC, are defined on the basis of the first two cysteines which are separated by one amino acid or are adjacent. Human CCL1, also well known as I-309, is a small glycoprotein (73 amino acid residue as mature form) secreted by activated T lymphocytes. Human I-309 is assumed to be homologous to the mouse TCA-3 despite of the only approximately 42% amino acid sequence identity. CCL1 interacts with the G protein-linked transmembrane chemokine receptors CCR8, and induces biochemical events that may result in the control of chemotaxis, proliferation, apoptosis and adhesion. It has been demonstrated that CCL1 displays chemotactic activity for monocytes and other cell types such as NK cells and dendritic cells, but not for neutrophils. Furthermore, as the only known physiological ligand for CCR8, CCL1 was identified as a potent inhibitor of HIV-1 envelope-mediated cell-cell fusion and virus infection.

References

1. Miller MD. et al., 1992, Proc Natl Acad Sci. 89: 2950-2954.
2. Roos RS. et al., 1997, J Biol Chem. 272: 17251-17254.
3. Bernardini G. et al., 2000, Blood. 96: 4039-4045.
4. Horuk R. et al., 1998, J Biol Chem. 273: 386-391.
5. Spinetti G. et al., 2003, J Leuko Biol. 73: 201-207.