Mouse CCDC80 Gene cDNA Clone / ORF Clone

CCDC80 cDNA Clone Datasheet

CCDC80 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_026439.2
• cDNA Size: 2850
• Gene Synonym: Urb; DRO1; Ssg1; 2610001E17Rik; Ccdc80
• Source: Mouse

CCDC80 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus coiled-coil domain containing 80 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for thirteen point mutations: 100 A/G resulting in the amino acid Thr substitution by Ala,344 G/A resulting in the amino acid Arg substitution by His, 888 G/C resulting in the amino acid Glu substitution by Asp, 1330 A/T resulting in the amino acid Thr substitution by Ser, 1366 G/A resulting in the amino acid Ala substitution by Thr,1442 C/T resulting in the amino acid Ala substitution by Val, 1520 A/C resulting in the amino acid Asp substitution by Ala, 1724 C/G resulting in the amino acid Thr substitution by Ser, 300 T/C, 1242 G/A, 1269 A/G, 1965 G/A and 2079 C/T not causing the amino acid variation.

CCDC80 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CCDC80 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CCDC80 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATGTGGA AAATGGGACC CCACTTCACC ACGCTCTTGG CCATGTGGCT GGTGTGTGGC
61       TCAGCATCTC ATTCTCCTGC CTTGGATAGC GACAGCCACG CTGGACGGAA AGTACCTTTG
121      GTTTCTCCAA TCAGCAGTAG GTCAGCTCGG TATCTGAGGC ACACCGGGAG GTCTGGTGGA
181      GTTGAGAAAT CCACCCAGGA AGAACCAAAT CCTCAGCCTT TCCAAAGGAG GAAGAGTGTA
241      CCAGTGTTGA GATTAGCTCA CCCGACCATG AGACCACCCC CCTCAGGTAT CAATGGAGTC
301      CCAGTCAGAC CTGAGGTGAG ACCCATAGCT AGGAGCTCGG CGCATGAGAT GGTCCGAGAT
361      GAGGGGTCTT CTGCTCGGAC AAGAATGTTG CGATTCCCCT CTGGATCTAG CTCTCCCAAT
421      ATCCTGGCCA GCTTTGCAGG AAAGAACAGG GTATGGGTCA TCTCAGCCCC TCACGCCTCA
481      GAGGGCTACT ACCGCCTCAT GATGAGCCTC CTGAAGGACG ACGTGTATTG TGAGCTGGCA
541      GAAAGGCACA TCCAACAGAT TGTGCTCTTC CACCAGGCAG GCGAGGAAGG GGGCAAGGTA
601      CGGAGGATCA CCAATGAAGG GCAGATCCTG GAGCAGCCTC TGGACCCCAA TCTCATCCCA
661      AAGCTGATGA GCTTCTTGAA ACTGGAGAAG GGCAAGTTTA GCATGGTGCT GTTAAAAAAG
721      ACTCTGCAGG TGGAGGAGCG CTACCCTTAC CCGGTCAGGC TGGAAGCCAT GTATGAGGTC
781      ATTGATCAAG GTCCCATCCG CAGGATCGAG AAAATCAGGC AGAAGGGTTT TGTCCAAAAG
841      TGTAAGGCCT CGGGCATAGA GGGCCATGTA GTCCAGGAAG GAAACGACGG CGGTGGAGGA
901      GCAGGAGGTA CAGGCCTGGG CGGTGATAAG AGGAAAGAGG ACCCAAGGAG AACACAAGTC
961      CACCCCACCA GAGAGGCTCC GAGAAAGCAG GCCACCAGCA AGGCAGCCAC TCCTCAGCCT
1021     CCCCCAACTC CAAGGGCCAC CACCCTTCCT CCTGCTCCAG TCACGACAGC CACTCGGGCC
1081     ACATCCCGGG TAGTGACAAT AGCCGCAAGA CCTACAACCA CCACTGCCTA TCCAGCTACC
1141     CAGAGGCCCT GGACATCCCG GTTGCATCCC TTCTCAGTCT CCCACAGGCC CCCTGCAACA
1201     GCAGAGGTGA CCACTGCCAG GGGCCCCTCC GTCTCAGAGC AACTATACCC TCTACCTCGG
1261     AAGGAGCAGC AGAGAGAGAA GCCACAGGCC ACCAGGAGGC CCAGCAAAGC CACCAACTAT
1321     GGAAGCTTCT CAGCCACCCC ACCTCCCACC CTCTGGGAGG TCAGCACAAG AGTTGTGGGC
1381     ACAAGCCGTT TCCGGGACAA CCGGACAGAC AAACGGGAAC ATGGCCATCA GGACCCAAAT
1441     GTGGTGCCAG GTCCTCACAA GCCAGTAAAG GGGAAGCTGC CCAAAAAGAA GGACAGAATT
1501     CTCAGCAACG AATATGAAGC CAAGTATGAC CTCAGCCAGC CCACCTCCTC TCAGGGGGAG
1561     GAGGAGCGGC AGGTGGATAG CGTTCCCTCG CAGAATGCCA AGGAGTCGAA AAAGCTTGAA
1621     AAGCTCGAGA AACCTGAGAA GGAGAAGAAG AAAAAAGGGA AGAGTGCAAA ACAAGACAAG
1681     TTACTCAAGA GTGAAAAGCA AGCGAAGAAA GCTGAGAAAA AGAGCAAACA GGAGAAAGAC
1741     AAGAATAAGA AGAAAAAGGC GGGTAAGACA GAGCAGGATG ACAATCAGAA GCCCACCGCA
1801     AAACATCTCG CTCCAAGTCC CAAGAAGTCA GTGGCCGACC TGTTGGGGTC TTTCGAAGGC
1861     AAACGAAGAC TCCTCCTGAT CACCACTCCC AAGGCTGAGA ACAATATGTA TGTGCAGCAG
1921     CGGGATGAAT ATCTGGAGAG CTTCTGCAAG ATGGCCACCA GGAGAATCTC TGTGGTCACC
1981     ATCTTTGGTC CTGTCAACAA CAGCTCCATG AAAATTGATC ACTTCCAGCT AGATAATGAG
2041     AAACCCATGC GTGTGGTGGA TGATGATGAC TTGGTGGATC AACATCTCAT CAGCGAGCTG
2101     AGGAAGGAGT ATGGAATGAC CTACGATGAC TTCTTCATGG TGCTGACAGA CGTGGATCTG
2161     AGAGTCAAGC AATACTACGA AGTGCCAATA GCAATGAAGT CCGTGTTTGA TCTTATTGAC
2221     ACTTTCCAAT CCCGAATCAA AGATATGGAG AAGCAGAAGA AGGAGGGCAT TGCCTGCAAG
2281     GAGGACAAGA GGCAGTCCCT GGAGAACTTC CTATCCAGGT TCCGATGGAG GAGGAGGTTG
2341     CTGGTGATCT CTGCTCCTAA TGATGAAGAC TGGGCCTATT CACAACAGCT CTCTGCCCTC
2401     AACGGTCAGG CATGCAATTT TGGTCTGCGA CATATAACCA TTCTGAAGCT TTTGGGTGTT
2461     GGAGAGGAAG TTGGAGGAGT TTTAGAACTG TTCCCAATTA ATGGGAGCTC CATCGTTGAG
2521     CGGGAAGATG TGCCAGCCCA CTTGGTCAAA GATATCCGCA ACTATTTTCA AGTGAGCCCA
2581     GAGTACTTCT CCATGCTTCT AGTTGGAAAA GATGGCAATG TTAAATCTTG GTATCCTTCT
2641     CCTATGTGGT CGATGGTCAT CGTGTATGAC TTAATTGATT CCATGCAACT TCGGAGACAG
2701     GAAATGGCCA TTCAGCAGTC TCTGGGGATG CGCTGCCCAG AAGATGAGTA TGCAGGATAT
2761     GGTTACCATA GTTATCACCA AGGATACCAG GATGGCTACC AGGATGACTA CCGTCATCAT
2821     GAAAGTTACC ACCATGGATA CCCTTACTGA

 

Quality Control:cDNA sequencing results is available upon request.