CAT / Catalase Protein (His Tag)

Catalase Protein Datasheet

CAT / Catalase Protein Product Information

• Synonym: MGC138422, MGC138424
• Protein Construction: A DNA sequence encoding the human CAT (P04040) (Ala 2-Leu 527) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

CAT / Catalase Protein QC Testing

• Purity: > 80 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human CAT consists of 545 amino acids and predicts a molecular mass of 61.9 kDa. It migrates as an approximately 60 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 50mM Tris, 100mM NaCl, pH8.0, 10% gly
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CAT / Catalase protein

CAT / Catalase Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CAT / Catalase Protein Description

Catalase is a ubiquitously expressed enzyme that catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a tetramer of four polypeptides chains containing four porphyrin heme groups that allow the enzyme to react with the hydrogen peroxide. The optimum PH of human catalase is approximately 7 and the optimum temperature is at 37 degree. Both the PH optimum and temperature for other catalases varies depending on the species. Catalase can be inhibited by a flux of O^2- generated in situ by the aerobic xanthine oxidase reaction. This inhibition of catalase by O^2- provides the basis for a synergism between superoxide dismutase and catalase.Such synergisms have been observed in vitro and may be significant in vivo. Catalase is used in the food industry for removing hydrogen peroxide from milk prior to cheese production. Another use is in food wrappers where it prevents food from oxidizing. Catalase is also used in the textile industry, removing hydrogen peroxide from fabrics to make sure the material is peroxide-free.

References

1. Schriner SE. et al., 2005, Science. 308 (5730): 1909-11.
2. Kono Y. et al., 1982, The Journal of Biological Chemistry. 257: 5751-4.