Anti-Catalase Antibody

Cat: 12084-T16
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All Catalase Reagents
Anti-Catalase Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Catalase Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human Catalase
Immunogen
Recombinant Human CAT / Catalase protein (Catalog#12084-H07B)
Preparation
Produced in rabbits immunized with purified, recombinant Human CAT / Catalase (rh CAT / Catalase; Catalog#12084-H07B; P04040; Ala2-Leu527). CAT / Catalase specific IgG was purified by Human CAT / Catalase affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Catalase Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Catalase Background Information

Catalase is a ubiquitously expressed enzyme that catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a tetramer of four polypeptides chains containing four porphyrin heme groups that allow the enzyme to react with the hydrogen peroxide. The optimum PH of human catalase is approximately 7 and the optimum temperature is at 37 degree. Both the PH optimum and temperature for other catalases varies depending on the species. Catalase can be inhibited by a flux of O2- generated in situ by the aerobic xanthine oxidase reaction. This inhibition of catalase by O2- provides the basis for a synergism between superoxide dismutase and catalase.Such synergisms have been observed in vitro and may be significant in vivo. Catalase is used in the food industry for removing hydrogen peroxide from milk prior to cheese production. Another use is in food wrappers where it prevents food from oxidizing. Catalase is also used in the textile industry, removing hydrogen peroxide from fabrics to make sure the material is peroxide-free.

Full Name
catalase
References
  • Schriner SE. et al., 2005, Science. 308 (5730): 1909-11.
  • Kono Y. et al., 1982, The Journal of Biological Chemistry. 257: 5751-4.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Catalase Antibody Rabbit Polyclonal ELISA 12084-RP01
    info info
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