Human CARTPT Gene cDNA Clone / ORF Clone

CARTPT cDNA Clone Datasheet

CARTPT cDNA Clone Product Information

• Gene Bank Ref.ID: BC029882
• cDNA Size: 351
• Gene Synonym: CART；CARTPT
• Source: Human

CARTPT cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens CART prepropeptide DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CARTPT cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CARTPT cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CARTPT cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGAGCT CCCGCGTGAG GCTGCTGCCC CTCCTGGGCG CCGCCCTGCT GCTGATGCTA
61       CCTCTGTTGG GTACCCGTGC CCAGGAGGAC GCCGAGCTCC AGCCCCGAGC CCTGGACATC
121      TACTCTGCCG TGGATGATGC CTCCCACGAG AAGGAGCTGA TCGAAGCGCT GCAAGAAGTC
181      TTGAAGAAGC TCAAGAGTAA ACGTGTTCCC ATCTATGAGA AGAAGTATGG CCAAGTCCCC
241      ATGTGTGACG CCGGTGAGCA GTGTGCAGTG AGGAAAGGGG CAAGGATCGG GAAGCTGTGT
301      GACTGTCCCC GAGGAACCTC CTGCAATTCC TTCCTCCTGA AGTGCTTATG A

 

Quality Control:cDNA sequencing results is available upon request.